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Literature summary for 2.7.11.1 extracted from

  • Alekseeva, M.; Mavletova, D.; Kolchina, N.; Nezametdinova, V.; Danilenko, V.
    Isolation and purification of recombinant serine/threonine protein kinases of the strain Bifidobacterium longum B379M and investigation of their activity (2015), Biochemistry, 80, 1303-1311 .
No PubMed abstract available
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
gene pkb1, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS in inclusion bodies Bifidobacterium longum
gene pkb2, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS in inclusion bodies Bifidobacterium longum
gene pkb3, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3)pLysS Bifidobacterium longum
gene pkb4, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3)pLysS Bifidobacterium longum
gene pkb5, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS in inclusion bodies Bifidobacterium longum
gene pkb6, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS in inclusion bodies Bifidobacterium longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34592
-
 mass spectrometry Bifidobacterium longum
51540
-
 mass spectrometry Bifidobacterium longum
76989
-
 mass spectroemtry Bifidobacterium longum
80070
-
 mass spectrometry Bifidobacterium longum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a protein Bifidobacterium longum
-
 ADP + a phosphoprotein
-
 r
ATP + a protein Bifidobacterium longum B379M
-
 ADP + a phosphoprotein
-
 r

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum M1QEG4
-
-
Bifidobacterium longum M1R338
-
-
Bifidobacterium longum M1R344
-
-
Bifidobacterium longum M1R969
-
-
Bifidobacterium longum M1RFK9
-
-
Bifidobacterium longum M1RNN3
-
-
Bifidobacterium longum B379M M1QEG4
-
-
Bifidobacterium longum B379M M1R338
-
-
Bifidobacterium longum B379M M1R344
-
-
Bifidobacterium longum B379M M1R969
-
-
Bifidobacterium longum B379M M1RFK9
-
-
Bifidobacterium longum B379M M1RNN3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme does not autophosphorylate Bifidobacterium longum
phosphoprotein the enzyme performs autophosphorylation Bifidobacterium longum

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Bifidobacterium longum
recombinant His-tagged isozyme, solubilized from Escherichia coli strain BL21(DE3)pLysS inclusion bodies, by nickel affinity chromatography Bifidobacterium longum
the recombinant His-tagged isozyme Pkb5 catalytic domain is purified and eluted from nickel affinity chromatography by a solution containing 6 M urea, 100 mM Na2HPO4, 10 mM Tris-HCl, 50 mM imidazole, pH 5.3, and 300 mM imidazole, followed by renaturing via dialysis against 1.5 M urea, 50 mM Tris-HCl, 200 mM NaCl, 10% glycerol, pH 8.0 Bifidobacterium longum

Renatured (Commentary)

Renatured (Comment) Organism
recombinant His-tagged isozyme Pkb1 from Escherichia coli inclusion bodies by denaturing lysis buffer, containing 6 M urea, 100 mM Na2HPO4, 10 mM Tris-HCl, pH 8.0, and dialysis Bifidobacterium longum
recombinant His-tagged isozyme Pkb2 from Escherichia coli inclusion bodies by denaturing lysis buffer, containing 6 M urea, 100 mM Na2HPO4, 10 mM Tris-HCl, pH 8.0, and dialysis Bifidobacterium longum
recombinant His-tagged isozyme Pkb5 catalytic domain from Escherichia coli inclusion bodies by denaturing lysis buffer, containing 6 M urea, 100 mM Na2HPO4, 10 mM Tris-HCl, pH 8.0 Bifidobacterium longum
recombinant His-tagged isozyme Pkb6 from Escherichia coli inclusion bodies by denaturing lysis buffer, containing 6 M urea, 100 mM Na2HPO4, 10 mM Tris-HCl, pH 8.0, and dialysis Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a protein
-
 Bifidobacterium longum ADP + a phosphoprotein
-
 r
ATP + a protein
-
 Bifidobacterium longum B379M ADP + a phosphoprotein
-
 r
additional information the enzyme performs autophosphorylation Bifidobacterium longum ?
-
 ?
additional information the enzyme does not perform autophosphorylation Bifidobacterium longum ?
-
 ?
additional information the enzyme's catalytic domain performs autophosphorylation Bifidobacterium longum ?
-
 ?
additional information the enzyme performs autophosphorylation Bifidobacterium longum B379M ?
-
 ?
additional information the enzyme does not perform autophosphorylation Bifidobacterium longum B379M ?
-
 ?
additional information the enzyme's catalytic domain performs autophosphorylation Bifidobacterium longum B379M ?
-
 ?

Subunits

Subunits Comment Organism
? 1 * 51540, mass spectrometry, 1 * 45000, recombinant enzyme, SDS-PAGE, 1 * 26766, enzyme catalytic domain, mass spectrometry Bifidobacterium longum
? x * 60439, mass spectrometry, x * 44000, recombinant enzyme, SDS-PAGE, x * 25916, enzyme catalytic domain, mass spectrometry Bifidobacterium longum
? x * 72260, mass spectrometry, x * 47000, recombinant enzyme, SDS-PAGE, x * 29344, enzyme catalytic domain, mass spectrometry Bifidobacterium longum
dimer 2 * 44000, recombinant enzyme, SDS-PAGE, 2 * 26334, enzyme catalytic domain, mass spectrometry Bifidobacterium longum
dimer 2 * 48000, recombinant enzyme, SDS-PAGE, 2 * 30363, enzyme catalytic domain, mass spectrometry Bifidobacterium longum
monomer 1 * 34592, mass spectrometry, 1 * 45000, recombinant enzyme, SDS-PAGE, 1 * 26705, enzyme catalytic domain, mass spectrometry Bifidobacterium longum

Synonyms

Synonyms Comment Organism
Pkb1
-
 Bifidobacterium longum
Pkb2
-
 Bifidobacterium longum
Pkb3
-
 Bifidobacterium longum
Pkb4
-
 Bifidobacterium longum
Pkb5
-
 Bifidobacterium longum
Pkb6
-
 Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 37 assay at room temperature or at 37°C Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bifidobacterium longum

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Bifidobacterium longum
ATP
-
 Bifidobacterium longum

pI Value

Organism Comment pI Value Maximum pI Value
Bifidobacterium longum isoelectric focusing
-
 4.87
Bifidobacterium longum isoelectric focusing
-
 5.14
Bifidobacterium longum isoelectric focusing
-
 5.34
Bifidobacterium longum isoelectric focusing
-
 5.54
Bifidobacterium longum isoelectric focusing
-
 6.32
Bifidobacterium longum isoelectric focusing
-
 6.97

General Information

General Information Comment Organism
physiological function serine/threonine protein kinases (STPK) are enzymes that modify other proteins by reversible phosphorylation at serine or threonine residues within consensus sequences, thereby modulating the activity of a broad range of enzymes and controlling specific protein-protein interactions. STPKs of Bifidobacterium can be elements of signal transduction networks and play major regulatory roles in bifidobacterial cells Bifidobacterium longum