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Literature summary for 2.7.11.1 extracted from

  • Fukasawa, T.; Enomoto, A.; Miyagawa, K.
    Serine-threonine kinase 38 regulates CDC25A stability and the DNA damage-induced G2/M checkpoint (2015), Cell. Signal., 27, 1569-1575 .
No PubMed abstract available
Search for more literature for 2.7.11.1

Activating Compound

Activating Compound Comment Organism Structure
H2O2
-
 Homo sapiens
additional information STK38 activity is stimulated by oxidative stresses such as hydrogen peroxide and X-ray irradiation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene STK38, recombinant expression in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme silencing by expression of short hairpin (sh) RNA targeting STK38, or negative control shRNA, in LU-99 cells via transfection by electroporation Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + CDC25A Homo sapiens STK38 phosphorylates CDC25A at Ser76 ADP + phospho-CDC25A
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q15208
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-
Lu-99 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CDC25A STK38 phosphorylates CDC25A at Ser76 Homo sapiens ADP + phospho-CDC25A
-
 ?
ATP + CDC25A recombinantly expressed full-length wild-type CDC25A or CDC25A mutants S124A and S76A are used as substrates. STK38 phosphorylates wild-type CDC25A and mutant S124A at Ser76, it shows no activity with the S76A mutant Homo sapiens ADP + phospho-CDC25A
-
 ?

Synonyms

Synonyms Comment Organism
NDR1
-
 Homo sapiens
serine-threonine kinase 38
-
 Homo sapiens
STK38
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
evolution STK38 is a member of the NDR family, which functions in cell-cyclerelated processes Homo sapiens
malfunction the cellular depletion of STK38 has no effect on the G1-phase transition under normal conditions, but leads to a slight increase in G1 arrest after X-ray irradiation. The depletion of STK38 alone may be insufficient to activate the G1 checkpoint. STK38 depletion has no effect on the distribution of cells in S-phase under normal conditions Homo sapiens
physiological function serine-threonine kinase 38 regulates CDC25A stability and the DNA damage-induced G2/M checkpoint. Enzyme STK38 is required for CDC25A degradation. Phosphorylation of Ser76 by STK38 regulates CDC25A's stability. Wild-type CDC25A is rapidly degraded, whereas the S76A mutant remains stable. STK38 is not involved in regulating the S-phase checkpoint, but enzyme CHK1 regulates the S-phase checkpoint by phosphorylating CDC25A at a number of sites, including Ser124. While CHK1 phosphorylates CDC25A at multiple sites, STK38's phosphorylation of CD25A may be more limited Homo sapiens