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Literature summary for 2.7.11.1 extracted from

  • Herhaus, L.; Perez-Oliva, A.; Cozza, G.; Gourlay, R.; Weidlich, S.; Campbell, D.; Pinna, L.; Sapkota, G.
    Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization (2015), Sci. Signal., 8, ra35.
No PubMed abstract available
Search for more literature for 2.7.11.1

Inhibitors

Inhibitors Comment Organism Structure
1-(beta-D-2'-deoxyribofuranosyl)-4,5,6,7-tetrabromo-1H-benzimidazole TDB, a cell-permeable selective inhibitor of CK2, inhibits the phosphorylation of OTUB1 at Ser16. In addition to CK2, TDB inhibits 3 other kinases, namely PIM1, CLK2 and DYRK1A, but none of these kinases phosphorylates OTUB1 in vitro Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + OTUB1 Homo sapiens OTUB1 is a tumor domain protease, phosphorylation at Ser16, in GSDSEGVN ADP + phospho-OTUB1
-
 ?
additional information Homo sapiens OTUB1 is a bona fide substrate for CK2 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + OTUB1 OTUB1 is a tumor domain protease, phosphorylation at Ser16, in GSDSEGVN Homo sapiens ADP + phospho-OTUB1
-
 ?
additional information OTUB1 is a bona fide substrate for CK2 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
tetramer the active CK2 can exist as a CK2alpha catalytic subunit monomer or a CK2alpha and non-catalytic, regulatory CK2beta subunit holoenzyme Homo sapiens

Synonyms

Synonyms Comment Organism
casein kinase 2
-
 Homo sapiens
CK2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction pharmacological inhibition or genetic ablation of CK2 blocks the phosphorylation of OTUB1 at Ser16 and its nuclear localization in various cells Homo sapiens
physiological function casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization and accumulation. OTUB1 is a member of the ovarian tumor domain protease (OTU) family of deubiquitylating enzymes (DUBs). DUBs are isopeptidases that remove attached ubiquitin chains or molecules from their targets. OTUB1 functions in deubiquitylating K48-linked ubiquitin chains as well as inhibiting the action of E2 ubiquitin-conjugating enzymes. The phosphorylation of OTUB1 at Ser16 does not alter its catalytic activity in vitro and the interactions with UBE2N. But the phosphorylation at Ser16 and subsequent nuclear localization of OTUB1 is essential for cells to repair ionizing radiation-induced DNA damage in osteosarcoma U2OS cells. Phosphorylation-dependent recruitment of OTUB1 to its other targets may also be likely. Phosphorylation of OTUB1 at Ser16 impacts IR-induced DNA-damage repair. Nuclear localization of OTUB1 may be necessary for DSB-induced 53BP1 foci formation Homo sapiens