Literature summary for 2.7.11.1 extracted from

An, N.; Lin, Y.; Mahajan, S.; Kellner, J.; Wang, Y.; Li, Z.; Kraft, A.; Kang, Y.
Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells (2013), Stem Cells, 31, 1202-1212 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene PIM1, recombinant overexpression in enzyme-deficient trangenic Pim1-Tx Mus musculus driven by vav hematopoietic promoter/regulatory elements. Compared to wild-type littermates, Pim1-Tx mice show enhanced hematopoiesis as demonstrated by increased numbers of Lin-Sca-1+c-Kit+ (LSK) hematopoietic stem/progenitor cells and cobblestone area forming cells, higher BrdU incorporation in long-term hematopoietic stem cell population, and a better ability to reconstitute lethally irradiated mice. Pim1-Tx mice express human PIM1 mRNA in all hematopoietic tissues including spleen, bone marrow and thymus, but not in non-hematopoietic tissues such as kidney	Homo sapiens

Cloned (Comment)

Organism

gene PIM1, recombinant overexpression in enzyme-deficient trangenic Pim1-Tx Mus musculus driven by vav hematopoietic promoter/regulatory elements. Compared to wild-type littermates, Pim1-Tx mice show enhanced hematopoiesis as demonstrated by increased numbers of Lin-Sca-1+c-Kit+ (LSK) hematopoietic stem/progenitor cells and cobblestone area forming cells, higher BrdU incorporation in long-term hematopoietic stem cell population, and a better ability to reconstitute lethally irradiated mice. Pim1-Tx mice express human PIM1 mRNA in all hematopoietic tissues including spleen, bone marrow and thymus, but not in non-hematopoietic tissues such as kidney

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	gene PIM1, recombinant overexpression in enzyme-deficient trangenic Pim1-Tx Mus musculus driven by vav hematopoietic promoter/regulatory elements. Compared to wild-type littermates, Pim1-Tx mice show enhanced hematopoiesis as demonstrated by increased numbers of Lin-Sca-1+c-Kit+ (LSK) hematopoietic stem/progenitor cells and cobblestone area forming cells, higher BrdU incorporation in long-term hematopoietic stem cell population, and a better ability to reconstitute lethally irradiated mice. Construction of Pim1-/-, Pim2-/-, Pim3-/- single knockout mice. hematopoietic stem cells from Pim1-/- KO mice show impaired long-term hematopoietic repopulating capacity in secondary and competitive transplantations. The defects are not observed in hematopoietic stem cells from Pim2-/- or Pim3-/- knockout mice. Limiting dilution competitive transplantation assay estimated that the frequency of LSKCD34. hematopoietic stem cells is reduced by about 28-fold in Pim1-/- KO mice compared to wild-type littermates. Approximately 10% of Pim1-Tx mice develope acute lymphoblastic leukemia/lymphoma between 141 and 435 days of life while wild-type littermates are leukemia free in entire lifetime. Hematopoiesis-associated genes are affected by Pim1 kinase. Phenotypes, detailed overview	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P11309	-	-
Mus musculus	P06803	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:B220+ cell	-	Homo sapiens	-
culture condition:GR1+ cell	low expression level	Homo sapiens	-
granulocyte	-	Homo sapiens	-
hematopoietic cell	-	Homo sapiens	-
hematopoietic cell	-	Mus musculus	-
hematopoietic stem cell	-	Homo sapiens	-
spleen	-	Mus musculus	-

Subunits

Subunits	Comment	Organism
?	x * 33000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
PIM1	-	Homo sapiens
PIM1	-	Mus musculus
Pim1 serine/threonine kinase	-	Homo sapiens
Pim1 serine/threonine kinase	-	Mus musculus
serine/	-	Homo sapiens
serine/	-	Mus musculus
threonine Pim kinase	-	Homo sapiens
threonine Pim kinase	-	Mus musculus

Expression

Organism	Comment	Expression
Mus musculus	Pim1 mRNA is upregulated by interleukin-3, interleukin-6 and TPO. In contrast, Pim2 and Pim3 mRNA expression is not changed with these cytokines	up

General Information

General Information	Comment	Organism
malfunction	several genes including Lef-1, Pax5 and Gata1 in hematopoietic stem cells that are affected by Pim1 deletion	Mus musculus
physiological function	important role of Pim1 kinase in regulating hematopoietic stem cell (HSC) cell proliferation and survival, and relative role of individual Pim kinase in hematopoietic stem cell functions and regulation, overview	Homo sapiens
physiological function	important role of Pim1 kinase in regulating hematopoietic stem cell (HSC) cell proliferation and survival, and relative role of individual Pim kinase in hematopoietic stem cell functions and regulation, overview. Pim1 might be associated with signal pathway(s) that is different from Pim2 or Pim3, thus having different roles in hematopoiesis	Mus musculus