Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | - |
Candida albicans | |
KN-62 | a Ca2+/calmodulin-dependent enzyme inhibitor | Candida albicans | |
additional information | no inhibition by H-7 and PKI | Candida albicans | |
W-7 | a Ca2+/calmodulin antagonist | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ka for calmodulin is 8.3 nM | Candida albicans | |
0.014 | - |
ATP | pH 7.0, 30°C | Candida albicans | |
0.02 | - |
syntide-2 | pH 7.0, 30°C | Candida albicans | |
0.063 | - |
Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu | pH 7.0, 30°C | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Candida albicans | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, activates | Candida albicans | |
Mg2+ | dependent on | Candida albicans | |
Mn2+ | partially stimulates | Candida albicans | |
additional information | little or no effect by Ni2+, Ba2+, Zn2+, Cu2+, and Cd2+ | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
1 * 69000, SDS-PAGE | Candida albicans |
71000 | - |
gel filtration | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
strain ATCC 3153, multifunctional enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme performs Ca2+/calmodulin-dependent autophosphorylation at a Ser residue | Candida albicans |
Purification (Comment) | Organism |
---|---|
644fold from cytosol by ammonium sulfate fractionation, DEAE cellulose ion exchange chromatography, and gel filtration | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0035 | - |
purified enzyme | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + bovine serum albumin | low activity | Candida albicans | ADP + phosphorylated bovine serum albumin | - |
? | |
ATP + casein | low activity | Candida albicans | ADP + phosphorylated casein | - |
? | |
ATP + histone IIS | - |
Candida albicans | ADP + phosphorylated histone IIS | - |
? | |
ATP + Kemptide | i.e. LRRASLG, low activity | Candida albicans | ADP + LRRAS(P)LG | - |
? | |
ATP + KKALRRQETVDAL | i.e. autocamtide-2 | Candida albicans | ADP + KKALRRQET(P)VDAL | - |
? | |
ATP + phosvitin | - |
Candida albicans | ADP + phosphorylated phosvitin | - |
? | |
ATP + protamin | low activity | Candida albicans | ADP + phosphorylated protamin | - |
? | |
ATP + syntide-2 | i.e. PLARTLSVAGLPGKK | Candida albicans | ADP + phosphorylated syntide-2 | - |
? | |
additional information | the enzyme performs Ca2+/calmodulin-dependent autophosphorylation at a Ser residue | Candida albicans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 69000, SDS-PAGE | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
Ca2+/CaMPK | - |
Candida albicans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Candida albicans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
cytosolic extract, 60 min, inactivation | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Candida albicans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
broad range | Candida albicans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Candida albicans |