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Literature summary for 2.7.11.25 extracted from

  • Cross, J.V.; Templeton, D.J.
    Oxidative stress inhibits MEKK1 by site-specific glutathionylation in the ATP-binding domain (2004), Biochem. J., 381, 675-683.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.25

Activating Compound

Activating Compound Comment Organism Structure
N-ethylmaleimide ASK1 activation at lower concentrations Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of MEKK1 and ASK1 in CV-1 cells using a vaccinia virus transfection system Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
glutathione binding to glutathione inhibits MEKK1 in vitro Rattus norvegicus
menadione i.e. 2-methyl-1,4-naphthoquinone, oxidative stress caused by menadione inhibits MEKK1, which can be reversed by DTT and glutathione, inhibition is thus caused by a reversible Cys1238 oxidation mechanism followed by binding to glutathione, no inhibition of MEKK1 mutant C1238V Rattus norvegicus
N-ethylmaleimide MEKK1 inhibition through Cys1238 alkylation, Cys1238 is located in the ATP binding domain, no inhibition of MEKK1 mutant C1238V Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus MEKK1 and ASK1 might play opposing roles in oxidative stress-induced activation of apoptosis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant MEKK1 and ASK1 by chitin affinity chromatography, endogenous MEKK1 by protein A affinity chromatography Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + SEK recombinant GST-tagged inactive KR-mutant SEK, i.e. SAPK/ERK or stress-activated protein kinase/extracellular-signal-regulated kinase, substrate Rattus norvegicus ADP + phosphorylated SEK
-
 ?
additional information MEKK1 and ASK1 might play opposing roles in oxidative stress-induced activation of apoptosis Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
apoptosis signal-regulated kinase 1
-
 Rattus norvegicus
ASK1
-
 Rattus norvegicus
MAPK/ERK kinase kinase 1
-
 Rattus norvegicus
MEKK1
-
 Rattus norvegicus
mitogen-activated protein kinase/extracellular-signal-regulated kinase kinase kinase 1
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus