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Literature summary for 2.7.13.3 extracted from

  • Stolov, A.; Valverde, A.; Ronald, P.; Burdman, S.
    Purification of soluble and active RaxH, a transmembrane histidine protein kinase from Xanthomonas oryzae pv. oryzae required for AvrXa21 activity (2007), Mol. Plant Pathol., 8, 93-101.
    View publication on PubMed

Application

Application Comment Organism
additional information induces phosphorylation of its concomitant response regulator RaxR Xanthomonas oryzae pv. oryzae

Cloned(Commentary)

Cloned (Comment) Organism
downstream of a histidine tag into vector pET15b, His-tagged full-length RaxH and RaxR overexpressed in Escherichia coli BL21 cells in the presence of sorbitol and glycine betaine to produce soluble His-RaxH Xanthomonas oryzae pv. oryzae

Protein Variants

Protein Variants Comment Organism
H222A lacks phosphorylation activities Xanthomonas oryzae pv. oryzae
additional information truncated His-RaxH, lacking the N-terminal transmembrane region, shows similar autophosphorylation activities than His-tagged full-length RaxH, but is unable to induce phosphorylation of His-RaxR Xanthomonas oryzae pv. oryzae
RD51A lacks phosphorylation activities Xanthomonas oryzae pv. oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31500
-
truncated His-tagged RaxH, predicted Xanthomonas oryzae pv. oryzae
48900
-
His-tagged RaxH, predicted Xanthomonas oryzae pv. oryzae

Organism

Organism UniProt Comment Textmining
Xanthomonas oryzae pv. oryzae
-
-
-
Xanthomonas oryzae pv. oryzae PXO99
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni2+-NTA resin Xanthomonas oryzae pv. oryzae

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl buffer, pH 7.650 mM KCl, 10 mM MgCl2, 0.1 mM DTT, 25–30% glycerol Xanthomonas oryzae pv. oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information full-length His-RaxH shows autophosphorylation activities Xanthomonas oryzae pv. oryzae ?
-
?
additional information full-length His-RaxH shows autophosphorylation activities Xanthomonas oryzae pv. oryzae PXO99 ?
-
?

Synonyms

Synonyms Comment Organism
histidine protein kinase
-
Xanthomonas oryzae pv. oryzae
RaxH
-
Xanthomonas oryzae pv. oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Xanthomonas oryzae pv. oryzae