Application | Comment | Organism |
---|---|---|
additional information | induces phosphorylation of its concomitant response regulator RaxR | Xanthomonas oryzae pv. oryzae |
Cloned (Comment) | Organism |
---|---|
downstream of a histidine tag into vector pET15b, His-tagged full-length RaxH and RaxR overexpressed in Escherichia coli BL21 cells in the presence of sorbitol and glycine betaine to produce soluble His-RaxH | Xanthomonas oryzae pv. oryzae |
Protein Variants | Comment | Organism |
---|---|---|
H222A | lacks phosphorylation activities | Xanthomonas oryzae pv. oryzae |
additional information | truncated His-RaxH, lacking the N-terminal transmembrane region, shows similar autophosphorylation activities than His-tagged full-length RaxH, but is unable to induce phosphorylation of His-RaxR | Xanthomonas oryzae pv. oryzae |
RD51A | lacks phosphorylation activities | Xanthomonas oryzae pv. oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31500 | - |
truncated His-tagged RaxH, predicted | Xanthomonas oryzae pv. oryzae |
48900 | - |
His-tagged RaxH, predicted | Xanthomonas oryzae pv. oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas oryzae pv. oryzae | - |
- |
- |
Xanthomonas oryzae pv. oryzae PXO99 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni2+-NTA resin | Xanthomonas oryzae pv. oryzae |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl buffer, pH 7.650 mM KCl, 10 mM MgCl2, 0.1 mM DTT, 2530% glycerol | Xanthomonas oryzae pv. oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | full-length His-RaxH shows autophosphorylation activities | Xanthomonas oryzae pv. oryzae | ? | - |
? | |
additional information | full-length His-RaxH shows autophosphorylation activities | Xanthomonas oryzae pv. oryzae PXO99 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
histidine protein kinase | - |
Xanthomonas oryzae pv. oryzae |
RaxH | - |
Xanthomonas oryzae pv. oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Xanthomonas oryzae pv. oryzae |