Cloned (Comment) | Organism |
---|---|
expresssion of GST-tagged wild-type and mutant enzymes, quantitative expression analysis, overview | Myxococcus xanthus |
Protein Variants | Comment | Organism |
---|---|---|
D696A | site-directed mutagenesis, the mutation of the receiver domain residue leads to release of phosphate to the medium, overview | Myxococcus xanthus |
H407A | site-directed mutagenesis, the mutation of the autophosphorylation site residue H407 inhibits autophosphorylation of the enzyme | Myxococcus xanthus |
additional information | construction of several mutants by in-frame deletions, espA mutants, encoding an orphan hybrid histidine kinase, alter the timing of the organism's developmental program, greatly accelerating developmental progression, phenotype, overview | Myxococcus xanthus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Myxococcus xanthus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
x * 83000, SDS-PAGE | Myxococcus xanthus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Myxococcus xanthus | Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program involves EspA, which plays a key role in the timing of expression of genes necessary for progression of cells through the developmental program, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myxococcus xanthus | Q9S434 | wild-type strain DZ2, gene espA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme autophosphorylates in vitro on the conserved histidine residue and then transfers the phosphoryl group to the conserved aspartate residue in the associated receiver domain | Myxococcus xanthus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | EspA developmental expression pattern, mutational analysis, overview | Myxococcus xanthus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program involves EspA, which plays a key role in the timing of expression of genes necessary for progression of cells through the developmental program, overview | Myxococcus xanthus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83000, SDS-PAGE | Myxococcus xanthus |
Synonyms | Comment | Organism |
---|---|---|
EspA | - |
Myxococcus xanthus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Myxococcus xanthus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Myxococcus xanthus |