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Literature summary for 2.7.13.3 extracted from

  • Higgs, P.I.; Jagadeesan, S.; Mann, P.; Zusman, D.R.
    EspA, an orphan hybrid histidine protein kinase, regulates the timing of expression of key developmental proteins of Myxococcus xanthus (2008), J. Bacteriol., 190, 4416-4426.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expresssion of GST-tagged wild-type and mutant enzymes, quantitative expression analysis, overview Myxococcus xanthus

Protein Variants

Protein Variants Comment Organism
D696A site-directed mutagenesis, the mutation of the receiver domain residue leads to release of phosphate to the medium, overview Myxococcus xanthus
H407A site-directed mutagenesis, the mutation of the autophosphorylation site residue H407 inhibits autophosphorylation of the enzyme Myxococcus xanthus
additional information construction of several mutants by in-frame deletions, espA mutants, encoding an orphan hybrid histidine kinase, alter the timing of the organism's developmental program, greatly accelerating developmental progression, phenotype, overview Myxococcus xanthus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Myxococcus xanthus 5829
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
x * 83000, SDS-PAGE Myxococcus xanthus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Myxococcus xanthus Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program involves EspA, which plays a key role in the timing of expression of genes necessary for progression of cells through the developmental program, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus Q9S434 wild-type strain DZ2, gene espA
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Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme autophosphorylates in vitro on the conserved histidine residue and then transfers the phosphoryl group to the conserved aspartate residue in the associated receiver domain Myxococcus xanthus

Source Tissue

Source Tissue Comment Organism Textmining
additional information EspA developmental expression pattern, mutational analysis, overview Myxococcus xanthus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program involves EspA, which plays a key role in the timing of expression of genes necessary for progression of cells through the developmental program, overview Myxococcus xanthus ?
-
?

Subunits

Subunits Comment Organism
? x * 83000, SDS-PAGE Myxococcus xanthus

Synonyms

Synonyms Comment Organism
EspA
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Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Myxococcus xanthus