Literature summary for 2.7.13.3 extracted from

Yeo, K.J.; Han, Y.H.; Eo, Y.; Cheong, H.K.
Expression, purification, crystallization and preliminary X-ray analysis of the extracellular sensory domain of DraK histidine kinase from Streptomyces coelicolor (2013), Acta Crystallogr. Sect. F, 69, 909-911.

Cloned(Commentary)

Cloned (Comment)	Organism
cloning of the extracellular sensory domain of DraK, consisting of 88 residues (Glu28-Arg115) from genomic DNA, and overexpression as N-terminally His6-tagged protein in Escherichia coli strain BL21(DE3)	Streptomyces coelicolor

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant N-terminally His6-tagged extracellular sensory domain of DraK, sitting drop vapour diffusion method, mixing 200 nl of of 12 mg/ml protein in 10 mM sodium acetate, pH 4.5, and 100 mM NaCl with reservoir solution containing 0.2 M potassium/sodium tartrate, and 20% PEG 3350, and equilibration against 0.070 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement	Streptomyces coelicolor

Crystallization (Comment)

Organism

purified recombinant N-terminally His6-tagged extracellular sensory domain of DraK, sitting drop vapour diffusion method, mixing 200 nl of of 12 mg/ml protein in 10 mM sodium acetate, pH 4.5, and 100 mM NaCl with reservoir solution containing 0.2 M potassium/sodium tartrate, and 20% PEG 3350, and equilibration against 0.070 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement

Streptomyces coelicolor

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	transmembrane protein	Streptomyces coelicolor	16020	-

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	Q9KZ83	-	-
Streptomyces coelicolor ATCC BAA-471D	Q9KZ83	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged extracellular sensory domain of DraK from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration	Streptomyces coelicolor

Subunits

Subunits	Comment	Organism
More	enzyme DraK consists of an extracellular domain flanked by two transmembrane helices (Leu5-Val27 and Leu126-Ile145), a sensory domain (Glu28-Thr125), a HAMP (histidine kinases, adenylyl cyclases, methyl-accepting chemotaxis proteins and phosphatases) domain (Asn149-Thr201) and a kinase domain (Ala202-Pro410) composed of His KA and CA domains	Streptomyces coelicolor

Synonyms

Synonyms	Comment	Organism
Drak	-	Streptomyces coelicolor
DraK histidine kinase	-	Streptomyces coelicolor

General Information

General Information	Comment	Organism
physiological function	DraK is a sensory histidine kinase involved in the differential regulation of antibiotics in Streptomyces coelicolor through the DraR/DraK two-component system	Streptomyces coelicolor