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Literature summary for 2.7.2.1 extracted from

  • Yoshimura, F.
    Purification and characterization of acetate kinase from Veillonella alcalescens ATCC 17748 (1978), Arch. Biochem. Biophys., 189, 424-432.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information succinate is unnecessary for the activity of this enzyme Veillonella parvula

Inhibitors

Inhibitors Comment Organism Structure
acetyl phosphate
-
Veillonella parvula
ADP
-
Veillonella parvula
HgCl2
-
Veillonella parvula
iodoacetamide
-
Veillonella parvula
N-ethylmaleimide
-
Veillonella parvula
p-chloromercuribenzoate
-
Veillonella parvula
phosphate
-
Veillonella parvula
potassium hydroxylamine
-
Veillonella parvula
sodium hydroxylamine
-
Veillonella parvula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
ADP pH 7.4, 37°C Veillonella parvula
1.2
-
acetyl phosphate pH 7.4, 37°C Veillonella parvula
2
-
ATP pH 7.4, 37°C Veillonella parvula
30
-
acetate pH 7.4, 37°C Veillonella parvula

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Veillonella parvula
Co2+ can replace Mg2+ Veillonella parvula
Mg2+
-
Veillonella parvula
Mn2+
-
Veillonella parvula
Pb2+
-
Veillonella parvula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE Veillonella parvula
66000
-
gel filtration Veillonella parvula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Veillonella parvula
-
ADP + acetyl phosphate
-
r

Organism

Organism UniProt Comment Textmining
Veillonella parvula
-
ATCC 17748
-

Purification (Commentary)

Purification (Comment) Organism
-
Veillonella parvula

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
948
-
-
Veillonella parvula

Storage Stability

Storage Stability Organism
-18°C, buffer containing 0.25 M sucrose, 40% of initial enzyme activity is lost within 2 months Veillonella parvula
-45°C, buffer containing 0.25 M sucrose, seems to be more stable than at -18°C Veillonella parvula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
Veillonella parvula ADP + acetyl phosphate
-
r
ATP + propionate
-
Veillonella parvula ADP + propionyl phosphate
-
r
GTP + acetate
-
Veillonella parvula GDP + acetyl phosphate
-
r
ITP + acetate
-
Veillonella parvula IDP + acetyl phosphate
-
r
additional information enzyme does not react with malate, maleate, bicarbonate, butyrate, tartrate, citrate, formate or succinate Veillonella parvula ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Veillonella parvula

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 with treatment at 50°C for 20 min 73% enzyme activity retained, treatment by heat at 60°C for 5 min results in 60% loss of activity, 70°C for 5 min results in complete loss of activity Veillonella parvula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 10
-
Veillonella parvula

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4 8 stable in buffers of pH 7.4 and 8.0, unstable under preincubation at pH 5.5 Veillonella parvula