Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | succinate is unnecessary for the activity of this enzyme | Veillonella parvula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl phosphate | - |
Veillonella parvula | |
ADP | - |
Veillonella parvula | |
HgCl2 | - |
Veillonella parvula | |
iodoacetamide | - |
Veillonella parvula | |
N-ethylmaleimide | - |
Veillonella parvula | |
p-chloromercuribenzoate | - |
Veillonella parvula | |
phosphate | - |
Veillonella parvula | |
potassium hydroxylamine | - |
Veillonella parvula | |
sodium hydroxylamine | - |
Veillonella parvula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ADP | pH 7.4, 37°C | Veillonella parvula | |
1.2 | - |
acetyl phosphate | pH 7.4, 37°C | Veillonella parvula | |
2 | - |
ATP | pH 7.4, 37°C | Veillonella parvula | |
30 | - |
acetate | pH 7.4, 37°C | Veillonella parvula |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Veillonella parvula | |
Co2+ | can replace Mg2+ | Veillonella parvula | |
Mg2+ | - |
Veillonella parvula | |
Mn2+ | - |
Veillonella parvula | |
Pb2+ | - |
Veillonella parvula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Veillonella parvula |
66000 | - |
gel filtration | Veillonella parvula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Veillonella parvula | - |
ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Veillonella parvula | - |
ATCC 17748 | - |
Purification (Comment) | Organism |
---|---|
- |
Veillonella parvula |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
948 | - |
- |
Veillonella parvula |
Storage Stability | Organism |
---|---|
-18°C, buffer containing 0.25 M sucrose, 40% of initial enzyme activity is lost within 2 months | Veillonella parvula |
-45°C, buffer containing 0.25 M sucrose, seems to be more stable than at -18°C | Veillonella parvula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Veillonella parvula | ADP + acetyl phosphate | - |
r | |
ATP + propionate | - |
Veillonella parvula | ADP + propionyl phosphate | - |
r | |
GTP + acetate | - |
Veillonella parvula | GDP + acetyl phosphate | - |
r | |
ITP + acetate | - |
Veillonella parvula | IDP + acetyl phosphate | - |
r | |
additional information | enzyme does not react with malate, maleate, bicarbonate, butyrate, tartrate, citrate, formate or succinate | Veillonella parvula | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Veillonella parvula |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | with treatment at 50°C for 20 min 73% enzyme activity retained, treatment by heat at 60°C for 5 min results in 60% loss of activity, 70°C for 5 min results in complete loss of activity | Veillonella parvula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 10 | - |
Veillonella parvula |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8 | stable in buffers of pH 7.4 and 8.0, unstable under preincubation at pH 5.5 | Veillonella parvula |