Any feedback?
Literature summary for 2.7.2.1 extracted from
- Acetate kinase from Methanosarcina thermophila, a key enzyme for methanogenesis (2005), Methods Biotechnol., 17, 239-246.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | protocol for overproduction of enzyme | Methanosarcina thermophila |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E97D | reduction of both Km and kcat-value | Methanosarcina thermophila |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | assay methods for both directions of reaction | Methanosarcina thermophila | |
| 2.8 | - |
acetate | mutant E97D, pH 7.5, 37°C | Methanosarcina thermophila |  |
| 22 | - |
acetate | wild-type, pH 7.5, 37°C | Methanosarcina thermophila | |
| 24 | - |
propionate | wild-type, pH 7.5, 37°C | Methanosarcina thermophila | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina thermophila | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl phosphate + hydroxylamine | - |
Methanosarcina thermophila | acetyl hydroxamate + phosphate | - |
r | |
| ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
| ATP + butyrate | 2% of reactivity with acetate | Methanosarcina thermophila | ADP + butyryl phosphate | - |
r | |
| ATP + ethanol | 1% of reactivity with acetate | Methanosarcina thermophila | ADP + ethyl phosphate | - |
r | |
| ATP + formate | 2% of reactivity with acetate | Methanosarcina thermophila | ADP + formyl phosphate | - |
r | |
| ATP + glycerol | 1% of reactivity with acetate | Methanosarcina thermophila | ADP + glycerol phosphate | - |
r | |
| ATP + glycine | 1% of reactivity with acetate | Methanosarcina thermophila | ADP + glycyl phosphate | - |
r | |
| ATP + glycolic acid | 4% of reactivity with acetate | Methanosarcina thermophila | ADP + ? | - |
r | |
| ATP + propionate | - |
Methanosarcina thermophila | ADP + propionyl phosphate | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 190 | - |
acetate | mutant E97D, pH 7.5, 37°C | Methanosarcina thermophila | |
| 1042 | - |
acetate | wild-type, pH 7.5, 37°C | Methanosarcina thermophila | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
