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Literature summary for 2.7.2.2 extracted from

  • Raijman, L.; Jones, M.E.
    Carbamate kinase (1973), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 9, 97-119.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Enterococcus faecalis

General Stability

General Stability Organism
2-mercaptoethanol protects against heat denaturation Mycoplasma hominis
80% loss of activity on dialysis against 0.04 M Tris, pH 8.5, 4°C, 18 h, Streptococcus lactis enzyme Lactococcus lactis
ammonium sulfate, 0.5 M, stabilizes against inactivation Enterococcus faecalis
unstable in dilute solutions, even at very low temperatures Neurospora crassa
unstable in dilute solutions, even at very low temperatures Lactococcus lactis
unstable in dilute solutions, even at very low temperatures Serratia marcescens
unstable in dilute solutions, even at very low temperatures Enterococcus faecalis
unstable in dilute solutions, even at very low temperatures Mycoplasma hominis

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Enterococcus faecalis
Cu2+
-
Enterococcus faecalis
iodoacetamide weak Enterococcus faecalis
p-hydroxymercuribenzoate
-
Enterococcus faecalis
Pb2+
-
Enterococcus faecalis
Silver-Tris
-
Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
acetate
-
Enterococcus faecalis

Metals/Ions

Metals/Ions Comment Organism Structure
Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Neurospora crassa
Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis
Co2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
Co2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
Co2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
Co2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis
Fe2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
Fe2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
Fe2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
Fe2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis
Mg2+
-
Neurospora crassa
Mg2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
Mg2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
Mg2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
Mg2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis
Mn2+
-
Neurospora crassa
Mn2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
Mn2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
Mn2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
Mn2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
2 * 31000, deduced from amino acid composition Enterococcus faecalis
40000 45300 sedimentation equilibrium method Enterococcus faecalis
61000
-
sucrose density gradient technique Mycoplasma hominis
66000
-
sedimentation data Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Neurospora crassa physiological role ?
-
?
additional information Lactococcus lactis physiological role ?
-
?
additional information Serratia marcescens physiological role ?
-
?
additional information Enterococcus faecalis physiological role ?
-
?
additional information Mycoplasma hominis physiological role ?
-
?
additional information Enterococcus faecalis D10 physiological role ?
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
D10
-
Enterococcus faecalis D10
-
D10
-
Lactococcus lactis
-
-
-
Mycoplasma hominis
-
type II strain 07
-
Neurospora crassa
-
-
-
Serratia marcescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterococcus faecalis

Reaction

Reaction Comment Organism Reaction ID
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Neurospora crassa
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Lactococcus lactis
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Serratia marcescens
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Enterococcus faecalis
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Mycoplasma hominis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay for forward and reverse reaction Neurospora crassa
additional information
-
assay for forward and reverse reaction Lactococcus lactis
additional information
-
assay for forward and reverse reaction Serratia marcescens
additional information
-
assay for forward and reverse reaction Enterococcus faecalis
additional information
-
assay for forward and reverse reaction Mycoplasma hominis
3100
-
-
Neurospora crassa
3100
-
-
Lactococcus lactis
3100
-
-
Serratia marcescens
3100
-
-
Enterococcus faecalis
3100
-
-
Mycoplasma hominis

Storage Stability

Storage Stability Organism
-20°C, 1 week, 50% loss of activity Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + carbamoyl phosphate
-
Neurospora crassa ATP + NH3 + CO2
-
r
ADP + carbamoyl phosphate
-
Lactococcus lactis ATP + NH3 + CO2
-
r
ADP + carbamoyl phosphate
-
Serratia marcescens ATP + NH3 + CO2
-
r
ADP + carbamoyl phosphate
-
Enterococcus faecalis ATP + NH3 + CO2
-
r
ADP + carbamoyl phosphate
-
Mycoplasma hominis ATP + NH3 + CO2
-
r
ADP + carbamoyl phosphate
-
Enterococcus faecalis D10 ATP + NH3 + CO2
-
r
ATP + NH3 + acetate
-
Enterococcus faecalis ADP + ?
-
?
ATP + NH3 + acetate
-
Enterococcus faecalis D10 ADP + ?
-
?
ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Neurospora crassa ADP + carbamoyl phosphate
-
r
ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Lactococcus lactis ADP + carbamoyl phosphate
-
r
ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Serratia marcescens ADP + carbamoyl phosphate
-
r
ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Enterococcus faecalis ADP + carbamoyl phosphate
-
r
ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Mycoplasma hominis ADP + carbamoyl phosphate
-
r
ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Enterococcus faecalis D10 ADP + carbamoyl phosphate
-
r
additional information no activity with GTP, ITP, UTP, CTP Lactococcus lactis ?
-
?
additional information some carbamate kinases also utilize acetate Neurospora crassa ?
-
?
additional information some carbamate kinases also utilize acetate Lactococcus lactis ?
-
?
additional information some carbamate kinases also utilize acetate Serratia marcescens ?
-
?
additional information some carbamate kinases also utilize acetate Enterococcus faecalis ?
-
?
additional information some carbamate kinases also utilize acetate Mycoplasma hominis ?
-
?
additional information physiological role Neurospora crassa ?
-
?
additional information physiological role Lactococcus lactis ?
-
?
additional information physiological role Serratia marcescens ?
-
?
additional information physiological role Enterococcus faecalis ?
-
?
additional information physiological role Mycoplasma hominis ?
-
?
additional information some carbamate kinases also utilize acetate Enterococcus faecalis D10 ?
-
?
additional information physiological role Enterococcus faecalis D10 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 31000, deduced from amino acid composition Enterococcus faecalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
2-mercaptoethanol protects against heat denaturation Mycoplasma hominis
60
-
2 min, 85% loss of activity Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
additional information biotin is not involved in reaction system Neurospora crassa
additional information biotin is not involved in reaction system Lactococcus lactis
additional information biotin is not involved in reaction system Serratia marcescens
additional information biotin is not involved in reaction system Enterococcus faecalis
additional information biotin is not involved in reaction system Mycoplasma hominis