Crystallization (Comment) | Organism |
---|---|
- |
Enterococcus faecalis |
General Stability | Organism |
---|---|
2-mercaptoethanol protects against heat denaturation | Mycoplasma hominis |
80% loss of activity on dialysis against 0.04 M Tris, pH 8.5, 4°C, 18 h, Streptococcus lactis enzyme | Lactococcus lactis |
ammonium sulfate, 0.5 M, stabilizes against inactivation | Enterococcus faecalis |
unstable in dilute solutions, even at very low temperatures | Neurospora crassa |
unstable in dilute solutions, even at very low temperatures | Lactococcus lactis |
unstable in dilute solutions, even at very low temperatures | Serratia marcescens |
unstable in dilute solutions, even at very low temperatures | Enterococcus faecalis |
unstable in dilute solutions, even at very low temperatures | Mycoplasma hominis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Enterococcus faecalis | |
Cu2+ | - |
Enterococcus faecalis | |
iodoacetamide | weak | Enterococcus faecalis | |
p-hydroxymercuribenzoate | - |
Enterococcus faecalis | |
Pb2+ | - |
Enterococcus faecalis | |
Silver-Tris | - |
Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
acetate | - |
Enterococcus faecalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Bivalent cation | required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Neurospora crassa | |
Bivalent cation | required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Lactococcus lactis | |
Bivalent cation | required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Serratia marcescens | |
Bivalent cation | required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Enterococcus faecalis | |
Bivalent cation | required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Mycoplasma hominis | |
Co2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Lactococcus lactis | |
Co2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Serratia marcescens | |
Co2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Enterococcus faecalis | |
Co2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Mycoplasma hominis | |
Fe2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Lactococcus lactis | |
Fe2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Serratia marcescens | |
Fe2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Enterococcus faecalis | |
Fe2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Mycoplasma hominis | |
Mg2+ | - |
Neurospora crassa | |
Mg2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Lactococcus lactis | |
Mg2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Serratia marcescens | |
Mg2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Enterococcus faecalis | |
Mg2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Mycoplasma hominis | |
Mn2+ | - |
Neurospora crassa | |
Mn2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Lactococcus lactis | |
Mn2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Serratia marcescens | |
Mn2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Enterococcus faecalis | |
Mn2+ | bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ | Mycoplasma hominis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000, deduced from amino acid composition | Enterococcus faecalis |
40000 | 45300 | sedimentation equilibrium method | Enterococcus faecalis |
61000 | - |
sucrose density gradient technique | Mycoplasma hominis |
66000 | - |
sedimentation data | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Neurospora crassa | physiological role | ? | - |
? | |
additional information | Lactococcus lactis | physiological role | ? | - |
? | |
additional information | Serratia marcescens | physiological role | ? | - |
? | |
additional information | Enterococcus faecalis | physiological role | ? | - |
? | |
additional information | Mycoplasma hominis | physiological role | ? | - |
? | |
additional information | Enterococcus faecalis D10 | physiological role | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
D10 | - |
Enterococcus faecalis D10 | - |
D10 | - |
Lactococcus lactis | - |
- |
- |
Mycoplasma hominis | - |
type II strain 07 | - |
Neurospora crassa | - |
- |
- |
Serratia marcescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterococcus faecalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O | mechanism | Neurospora crassa | |
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O | mechanism | Lactococcus lactis | |
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O | mechanism | Serratia marcescens | |
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O | mechanism | Enterococcus faecalis | |
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O | mechanism | Mycoplasma hominis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay for forward and reverse reaction | Neurospora crassa |
additional information | - |
assay for forward and reverse reaction | Lactococcus lactis |
additional information | - |
assay for forward and reverse reaction | Serratia marcescens |
additional information | - |
assay for forward and reverse reaction | Enterococcus faecalis |
additional information | - |
assay for forward and reverse reaction | Mycoplasma hominis |
3100 | - |
- |
Neurospora crassa |
3100 | - |
- |
Lactococcus lactis |
3100 | - |
- |
Serratia marcescens |
3100 | - |
- |
Enterococcus faecalis |
3100 | - |
- |
Mycoplasma hominis |
Storage Stability | Organism |
---|---|
-20°C, 1 week, 50% loss of activity | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + carbamoyl phosphate | - |
Neurospora crassa | ATP + NH3 + CO2 | - |
r | |
ADP + carbamoyl phosphate | - |
Lactococcus lactis | ATP + NH3 + CO2 | - |
r | |
ADP + carbamoyl phosphate | - |
Serratia marcescens | ATP + NH3 + CO2 | - |
r | |
ADP + carbamoyl phosphate | - |
Enterococcus faecalis | ATP + NH3 + CO2 | - |
r | |
ADP + carbamoyl phosphate | - |
Mycoplasma hominis | ATP + NH3 + CO2 | - |
r | |
ADP + carbamoyl phosphate | - |
Enterococcus faecalis D10 | ATP + NH3 + CO2 | - |
r | |
ATP + NH3 + acetate | - |
Enterococcus faecalis | ADP + ? | - |
? | |
ATP + NH3 + acetate | - |
Enterococcus faecalis D10 | ADP + ? | - |
? | |
ATP + NH3 + CO2 | reaction proceeds more readily in direction of ATP synthesis | Neurospora crassa | ADP + carbamoyl phosphate | - |
r | |
ATP + NH3 + CO2 | reaction proceeds more readily in direction of ATP synthesis | Lactococcus lactis | ADP + carbamoyl phosphate | - |
r | |
ATP + NH3 + CO2 | reaction proceeds more readily in direction of ATP synthesis | Serratia marcescens | ADP + carbamoyl phosphate | - |
r | |
ATP + NH3 + CO2 | reaction proceeds more readily in direction of ATP synthesis | Enterococcus faecalis | ADP + carbamoyl phosphate | - |
r | |
ATP + NH3 + CO2 | reaction proceeds more readily in direction of ATP synthesis | Mycoplasma hominis | ADP + carbamoyl phosphate | - |
r | |
ATP + NH3 + CO2 | reaction proceeds more readily in direction of ATP synthesis | Enterococcus faecalis D10 | ADP + carbamoyl phosphate | - |
r | |
additional information | no activity with GTP, ITP, UTP, CTP | Lactococcus lactis | ? | - |
? | |
additional information | some carbamate kinases also utilize acetate | Neurospora crassa | ? | - |
? | |
additional information | some carbamate kinases also utilize acetate | Lactococcus lactis | ? | - |
? | |
additional information | some carbamate kinases also utilize acetate | Serratia marcescens | ? | - |
? | |
additional information | some carbamate kinases also utilize acetate | Enterococcus faecalis | ? | - |
? | |
additional information | some carbamate kinases also utilize acetate | Mycoplasma hominis | ? | - |
? | |
additional information | physiological role | Neurospora crassa | ? | - |
? | |
additional information | physiological role | Lactococcus lactis | ? | - |
? | |
additional information | physiological role | Serratia marcescens | ? | - |
? | |
additional information | physiological role | Enterococcus faecalis | ? | - |
? | |
additional information | physiological role | Mycoplasma hominis | ? | - |
? | |
additional information | some carbamate kinases also utilize acetate | Enterococcus faecalis D10 | ? | - |
? | |
additional information | physiological role | Enterococcus faecalis D10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31000, deduced from amino acid composition | Enterococcus faecalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
2-mercaptoethanol protects against heat denaturation | Mycoplasma hominis |
60 | - |
2 min, 85% loss of activity | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | biotin is not involved in reaction system | Neurospora crassa | |
additional information | biotin is not involved in reaction system | Lactococcus lactis | |
additional information | biotin is not involved in reaction system | Serratia marcescens | |
additional information | biotin is not involved in reaction system | Enterococcus faecalis | |
additional information | biotin is not involved in reaction system | Mycoplasma hominis |