Literature summary for 2.7.2.3 extracted from

Davies, G.J.; Gamblin, S.J.; Littlechild, J.A.; Watson, H.C.
Purification, crystallization and preliminary X-ray analysis of the 3-phosphoglycerate kinase from Bacillus stearothermophilus (1992), J. Mol. Biol., 227, 1263-1264.

Search for more literature for 2.7.2.3

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour phase diffusion method, 18°C, protein solution: 15 mg/ml, Tris-HCl, pH 7.0, 1 mM MgATP2-, 0.1 mM NaN3, 10% w/v polyethylene glycol 600, precipitant is 40% w/v polyethylene glycol 600, X-ray diffraction structure determination and analysis	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	NCA 1503	-
Geobacillus stearothermophilus NCA 1503	-	NCA 1503	-

Purification (Commentary)

Purification (Comment)	Organism
-	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 3-phospho-D-glycerate	-	Geobacillus stearothermophilus	ADP + 1,3-diphosphoglycerate	-	r
ATP + 3-phospho-D-glycerate	-	Geobacillus stearothermophilus NCA 1503	ADP + 1,3-diphosphoglycerate	-	r

Cofactor

Cofactor	Comment	Organism	Structure
ADP	-	Geobacillus stearothermophilus
ATP	required as phosphate donor	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)