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Literature summary for 2.7.2.3 extracted from
- Separation, purification, and comparative properties of chloroplast and cytoplasmic phosphoglycerate kinase from barley leaves (1990), Plant Physiol., 93, 374-383.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | 90% of total enzyme activity | Hordeum vulgare | 9507 | - |
| cytosol | 10% of total enzyme activity | Hordeum vulgare | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
cytoplasmic and chloroplastic isoenzyme, gel filtration | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
2 isozymes, cytosolic and chloroplastic | - |
| Hordeum vulgare | - |
cv. Golden Promise | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 14 days old leaves, 545fold purification of the chloroplastic and 530fold of the cytoplasmic isoenzyme | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Hordeum vulgare | - |
| seedling | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 69 | - |
purified cytosolic isozyme | Hordeum vulgare |
| 644 | - |
purified chloroplastic isozyme | Hordeum vulgare |
| 914 | - |
purified isozymes | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Hordeum vulgare | ADP + 1,3-diphosphoglycerate | - |
r |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000, chloroplastic and cytoplasmic enzyme | Hordeum vulgare |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Hordeum vulgare |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Hordeum vulgare | |
| ATP | required as phosphate donor | Hordeum vulgare | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hordeum vulgare | chloroplastic isozyme, isoelectric focusing | - |
5.2 |
| Hordeum vulgare | cytsolic isozyme, isoelectric focusing | - |
5.4 |
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Release 2023.1 (January 2023)
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