Crystallization (Comment) | Organism |
---|---|
crystallization from ammonium sulfate precipitation overnight with gradually raising of temperature from 4°C to room temperature, in presence of ATP | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | strong | Thermus thermophilus | |
2,3-diphosphoglycerate | - |
Thermus thermophilus | |
ADP | - |
Thermus thermophilus | |
CTP | competitive to ATP | Thermus thermophilus | |
GTP | competitive to ATP | Thermus thermophilus | |
ITP | weak competitive inhibitor | Thermus thermophilus | |
NaCl | weak | Thermus thermophilus | |
UTP | weak | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
ATP | pH 7.5, 22°C | Thermus thermophilus | |
1.79 | - |
3-phospho-D-glycerate | pH 7.5, 22°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermus thermophilus | |
Mg2+ | optimum concentration: 8-16 mM | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
1 * 44000, SDS-PAGE | Thermus thermophilus |
44600 | - |
sedimentation equilibrium studies | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
259 | - |
purified enzyme | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | high specificity for ATP | Thermus thermophilus | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | high specificity for ATP | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + 1,3-diphosphoglycerate | - |
r | |
CTP + 3-phospho-D-glycerate | low activity | Thermus thermophilus | CDP + 1,3-diphosphoglycerate | - |
? | |
CTP + 3-phospho-D-glycerate | low activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | CDP + 1,3-diphosphoglycerate | - |
? | |
GTP + 3-phospho-D-glycerate | low activity | Thermus thermophilus | GDP + 1,3-diphosphoglycerate | - |
? | |
GTP + 3-phospho-D-glycerate | low activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | GDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | - |
Thermus thermophilus | IDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | IDP + 1,3-diphosphoglycerate | - |
? | |
UTP + 3-phospho-D-glycerate | no activity | Thermus thermophilus | UDP + 1,3-diphosphoglycerate | - |
? | |
UTP + 3-phospho-D-glycerate | no activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | UDP + 1,3-diphosphoglycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Thermus thermophilus |
More | secondary structure: 29% alpha-helix, 11% pleated sheets | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
79 | - |
20 min, no loss of activity | Thermus thermophilus |
92 | - |
5 min, 75% loss of activity | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 10 | pH 4.8: about 45% of activity maximum, pH 10.0: about 90% of activity maximum | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Thermus thermophilus | |
ATP | required as phosphate donor | Thermus thermophilus | |
CTP | - |
Thermus thermophilus | |
GTP | - |
Thermus thermophilus | |
ITP | - |
Thermus thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermus thermophilus | analytical electric focusing | - |
5 |