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Literature summary for 2.7.2.3 extracted from

  • Pegorado, B.; Lee, C.Y.
    Purification and characterization of two isozymes of 3-phosphoglycerate kinase from the mouse (1978), Biochim. Biophys. Acta, 522, 423-433.
    View publication on PubMed
Search for more literature for 2.7.2.3

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate
-
 Mus musculus
N-ethylmaleimide
-
 Mus musculus
Urea
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pH-dependence of Km values for the substrates in forward and reverse reaction, 2 isozymes Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Mus musculus
45000
-
 isozymes PGKA and PGKB Mus musculus
47000
-
 1 * 47000, isozymes PGKA and PGKB, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 2 isozymes PGKA and PGKB
-
Mus musculus
-
 DBA/2J
-
Mus musculus DBA/2J
-
 DBA/2J
-

Purification (Commentary)

Purification (Comment) Organism
isozymes PGKA and PGKB Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle isozyme PGKA Mus musculus
-
testis isozyme PGKB Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
260 450 purified isozyme PGKB Mus musculus
500
-
 purified isozyme PGKA Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
 Mus musculus ADP + 1,3-diphosphoglycerate
-
 r
ATP + 3-phospho-D-glycerate
-
 Mus musculus DBA/2J ADP + 1,3-diphosphoglycerate
-
 r
CTP + 3-phospho-D-glycerate 1% of the activity with ATP Mus musculus CDP + 1,3-diphosphoglycerate
-
 ?
CTP + 3-phospho-D-glycerate 1% of the activity with ATP Mus musculus DBA/2J CDP + 1,3-diphosphoglycerate
-
 ?
dATP + 3-phospho-D-glycerate
-
 Mus musculus dADP + 1,3-diphosphoglycerate
-
 ?
dATP + 3-phospho-D-glycerate
-
 Mus musculus DBA/2J dADP + 1,3-diphosphoglycerate
-
 ?
GTP + 3-phospho-D-glycerate 60% of the activity with ATP Mus musculus GDP + 1,3-diphosphoglycerate
-
 ?
GTP + 3-phospho-D-glycerate 60% of the activity with ATP Mus musculus DBA/2J GDP + 1,3-diphosphoglycerate
-
 ?
UTP + 3-phospho-D-glycerate 1% of the activity with ATP Mus musculus UDP + 1,3-diphosphoglycerate
-
 ?
UTP + 3-phospho-D-glycerate 1% of the activity with ATP Mus musculus DBA/2J UDP + 1,3-diphosphoglycerate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, isozymes PGKA and PGKB, SDS-PAGE Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Mus musculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 activity of both isozymes in either reaction direction increases steadily with increasing temperature within this range Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 6.0, 10 min: 20% loss of activity, PGKA, 80% loss of activity PGKB Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Mus musculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.7
-
 isozyme PGKB: loss of 70% activity after 45 min, isozyme PGKA: loss of 10% activity after 45 min Mus musculus
9.6
-
 isozymes PGKA and PGKB: stable for at least 30 min Mus musculus

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Mus musculus
ATP required as phosphate donor Mus musculus
CTP
-
 Mus musculus
dATP
-
 Mus musculus
GTP
-
 Mus musculus
UTP
-
 Mus musculus