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Literature summary for 2.7.2.3 extracted from

  • Bernstein, B.E.; Hol, W.G.
    Crystal structures of substrates and products bound to the phosphoglycerate kinase active site reveal the catalytic mechanism (1998), Biochemistry, 37, 4429-4436.
    View publication on PubMed
Search for more literature for 2.7.2.3

Crystallization (Commentary)

Crystallization (Comment) Organism
purified, recombinant enzyme is crystallized as a ternary complex by vapour diffusion method, 10°C, protein solution: 6 mg/ml, 10 mM 3-phospho-D-glycerate, 10 mM MgADP, 25 mM Tris, pH 7.5, 50 mM NaCl, 10 m DTT, plus equal volume of 2.5 M sodium potassium phosphate, pH 8.0 Trypanosoma brucei
purified, recombinant enzyme is crystallized in complex with nucleotide analogue Mg-beta,gamma-imido-adenosine-5'-triphosphate by vapour diffusion method, 10°C, protein solution: 6 mg/ml, 10 mM 3-phospho-D-glycerate, 10 mM MgADP-, 25 mM Tris, pH 7.5, 50 mM NaCl, 10 m DTT, plus equal volume of 2.5 M sodium potassium phosphate, pH 7.5 Trypanosoma brucei
structure determination and analysis Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 ternary complex of 2 dimers build of 4 enzyme molecules, crystal structure determination Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate mechanism Trypanosoma brucei
a
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate substrate binding Trypanosoma brucei
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
 Trypanosoma brucei ADP + 1,3-diphosphoglycerate
-
 r

Subunits

Subunits Comment Organism
More ternary structure Trypanosoma brucei
More structure model Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Trypanosoma brucei
ATP true substrate is the magnesium complexes of ATP Trypanosoma brucei
ATP required as phosphate donor Trypanosoma brucei