Cloned (Comment) | Organism |
---|---|
expression of the glycosomal enzyme lacking the C-terminal glycosome-specific 20-residue signal sequence, in Escherichia coli | Trypanosoma brucei |
overexpression of glycosomal and cytosolic isozymes in Escherichia coli BL21 | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ib1 | isozymes are differntly sensitive | Trypanosoma brucei | |
suramin | isozymes are differently sensitive | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
ATP | recombinant cytosolic isozyme, pH 7.6, 25°C | Trypanosoma brucei | |
0.28 | - |
ATP | native glycosomal isozyme, pH 7.6, 25°C | Trypanosoma brucei | |
0.29 | - |
ATP | recombinant glycosomal isozyme, pH 7.6, 25°C | Trypanosoma brucei | |
0.33 | - |
ATP | recombinant truncated glycosomal isozyme, pH 7.6, 25°C | Trypanosoma brucei | |
0.46 | - |
ATP | native glycosomal isozyme, pH 7.6, 25°C | Trypanosoma brucei | |
1.25 | - |
3-phospho-D-glycerate | native cytosolic isozyme, pH 7.6, 25°C | Trypanosoma brucei | |
1.55 | - |
3-phospho-D-glycerate | native glycosomal isozyme, pH 7.6, 25°C | Trypanosoma brucei | |
2 | - |
3-phospho-D-glycerate | recombinant glycosomal isozyme and native cytosoli isozyme, pH 7.6, 25°C | Trypanosoma brucei | |
11.9 | - |
3-phospho-D-glycerate | recombinant truncated glycosomal isozyme, pH 7.6, 25°C | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic isozyme | Trypanosoma brucei | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, cytosolic and truncated glycosomal isozymes, SDS-PAGE | Trypanosoma brucei |
47000 | - |
x * 47000, glycosomal isozyme, SDS-PAGE | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
glycosomal PGKA and cytosolic PGKB isozymes | - |
Purification (Comment) | Organism |
---|---|
2 isozymes and the truncated glycosomal isozyme, recombinant from Escherichia coli, to near homogeneity | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
159 | - |
purified recombinant truncated glycosomal isozyme | Trypanosoma brucei |
159 | - |
purified recombinant cytosolic isozyme | Trypanosoma brucei |
1311 | - |
purified recombinant glycosomal isozyme | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Trypanosoma brucei | ADP + 1,3-diphosphoglycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, glycosomal isozyme, SDS-PAGE | Trypanosoma brucei |
dimer | x * 45000, cytosolic and truncated glycosomal isozymes, SDS-PAGE | Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Trypanosoma brucei | |
ATP | required as phosphate donor | Trypanosoma brucei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trypanosoma brucei | native cytosolic isozyme, isoelectric focusing | 6.8 | 6.3 |
Trypanosoma brucei | recombinant cytosolic isozyme, isoelectric focussing | - |
6.5 |
Trypanosoma brucei | truncated glycosomal isozyme, isoelectric focusing | - |
9.2 |
Trypanosoma brucei | glycosomal isozyme, isoelectric focusing | - |
9.3 |