Cloned (Comment) | Organism |
---|---|
gene pgk, DNA and amino acid sequence determination, overexpression as native and His-tagged wild-type enzyme in Escherichia coli BL21(DE3), the His-tag strongly alters the enzymes properties, e.g. thermostability, kinetic parameters like kcat and Km | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SO42- | above 50 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
ATP | recombinant wild-type enzyme, pH 7.5, 25°C | Pseudomonas sp. | |
0.24 | - |
ATP | native wild-type enzyme, pH 7.5, 25°C | Pseudomonas sp. | |
0.38 | - |
ATP | recombinant His-tagged enzyme, pH 7.5, 25°C | Pseudomonas sp. | |
0.48 | - |
3-phospho-D-glycerate | native wild-type enzyme, pH 7.5, 25°C | Pseudomonas sp. | |
0.53 | - |
3-phospho-D-glycerate | recombinant wild-type enzyme, pH 7.5, 25°C | Pseudomonas sp. | |
0.89 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 25°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant wild-type, SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q9RBS3 | gene pgk, strain TACII18 | - |
Purification (Comment) | Organism |
---|---|
native wild-type from Pseudomonas sp., and recombinant native and His-tagged enzyme from Escherichia coli as , to homogeneity | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
550 | - |
purified recombinant His-tagged enzyme | Pseudomonas sp. |
750 | - |
purified recombinant wild-type and native wild-type enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Pseudomonas sp. | ADP + 1,3-diphosphoglycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant wild-type, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant His-tagged wild-type enzyme shows reduced thermostability compared to the native and recombinant wild-type without tag | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | temperature-dependence of kinetic | Pseudomonas sp. | |
362 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme | Pseudomonas sp. | |
411 | - |
ATP | recombinant His-tagged enzyme | Pseudomonas sp. | |
500 | - |
ATP | recombinant wild-type enzyme | Pseudomonas sp. | |
519 | - |
ATP | native wild-type enzyme | Pseudomonas sp. | |
537 | - |
3-phospho-D-glycerate | recombinant wild-type enzyme | Pseudomonas sp. | |
540 | - |
3-phospho-D-glycerate | native wild-type enzyme | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas sp. |
8 | - |
recombinant wild-type | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Pseudomonas sp. | |
ATP | required as phosphate donor | Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | recombinant wild-type | - |
4.9 |