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Literature summary for 2.7.2.3 extracted from

  • Bentahir, M.; Feller, G.; Aittaleb, M.; Lamotte-Brasseur, J.; Himri, T.; Chessa, J.P.; Gerday, C.
    Structural, kinetic, and calorimetric characterization of the cold-active phosphoglycerate kinase from the antarctic Pseudomonas sp. TACII18 (2000), J. Biol. Chem., 275, 11147-11153.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pgk, DNA and amino acid sequence determination, overexpression as native and His-tagged wild-type enzyme in Escherichia coli BL21(DE3), the His-tag strongly alters the enzymes properties, e.g. thermostability, kinetic parameters like kcat and Km Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
SO42- above 50 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
ATP recombinant wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
0.24
-
ATP native wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
0.38
-
ATP recombinant His-tagged enzyme, pH 7.5, 25°C Pseudomonas sp.
0.48
-
3-phospho-D-glycerate native wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
0.53
-
3-phospho-D-glycerate recombinant wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
0.89
-
3-phospho-D-glycerate recombinant His-tagged enzyme, pH 7.5, 25°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, recombinant wild-type, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q9RBS3 gene pgk, strain TACII18
-

Purification (Commentary)

Purification (Comment) Organism
native wild-type from Pseudomonas sp., and recombinant native and His-tagged enzyme from Escherichia coli as , to homogeneity Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
550
-
purified recombinant His-tagged enzyme Pseudomonas sp.
750
-
purified recombinant wild-type and native wild-type enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
Pseudomonas sp. ADP + 1,3-diphosphoglycerate
-
r

Subunits

Subunits Comment Organism
? x * 40000, recombinant wild-type, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the recombinant His-tagged wild-type enzyme shows reduced thermostability compared to the native and recombinant wild-type without tag Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information temperature-dependence of kinetic Pseudomonas sp.
362
-
3-phospho-D-glycerate recombinant His-tagged enzyme Pseudomonas sp.
411
-
ATP recombinant His-tagged enzyme Pseudomonas sp.
500
-
ATP recombinant wild-type enzyme Pseudomonas sp.
519
-
ATP native wild-type enzyme Pseudomonas sp.
537
-
3-phospho-D-glycerate recombinant wild-type enzyme Pseudomonas sp.
540
-
3-phospho-D-glycerate native wild-type enzyme Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas sp.
8
-
recombinant wild-type Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
ADP
-
Pseudomonas sp.
ATP required as phosphate donor Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. recombinant wild-type
-
4.9