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Literature summary for 2.7.2.3 extracted from
- Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis (2000), Mol. Biochem. Parasitol., 108, 207-216.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme of parasite is target for vaccine and drug development, enzyme elicits antibodies and can therefore be utilized as immunoreagent in serodiagnostics for clonorchiasis | Clonorchis sinensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned from genetic library, DNA sequence determination and analysis, expression in Escherichia coli as glutathione-S-transferase-fusion protein, which is subsequently cleaved off with thrombin | Clonorchis sinensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Clonorchis sinensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clonorchis sinensis | P50311 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant as glutathione-S-transferase-tagged enzyme from Escherichia coli, to homogeneity | Clonorchis sinensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 530 | - |
purified, detagged recombinant enzyme | Clonorchis sinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Clonorchis sinensis | ADP + 1,3-diphosphoglycerate | - |
r |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Clonorchis sinensis |
| More | secondary structure | Clonorchis sinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Clonorchis sinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Clonorchis sinensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Clonorchis sinensis | |
| ATP | required as phosphate donor | Clonorchis sinensis | |
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