Application | Comment | Organism |
---|---|---|
additional information | not resistant to proteolytic cleavage, both domains of the yeast protein fold in isolation into stable structures, extremely similar tertiary structures and global stabilities as compared to the homologous Escherichia coli PGK, but significant differences in proteolysis | Saccharomyces cerevisiae |
additional information | resistant to proteolytic cleavage, the C-domain of PGK cannot be expressed or fold independently of the N-domain, extremely similar tertiary structures and global stabilities as compared to the homologous Saccharomyces cerevisiae PGK, but significant differences in proteolysis | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
into vector pET27b and expressed in Escherichia coli BL21 (DE3)pLysS cells | Saccharomyces cerevisiae |
into vector pET27b and expressed in Escherichia coli BL21(DE3)pLysS cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in the presence of 1 mM AMP-PNP and 1 mM MgCl2, by the vapor diffusion method, solved by molecular replacement to 2.4 A resolution | Escherichia coli |
vapor diffusion method | Escherichia coli |
General Stability | Organism |
---|---|
phosphoglycerate kinase is resistant to proteolytic cleavage. ecPGK can only be digested through its globally unfolded state, suggesting that cooperativity between the two domains results in a more rigid protein that has low susceptibility to proteolysis | Escherichia coli |
the extrapolated unfolding rate of Escherichia coli PGK is 100000 slower than that of the yeast homolog | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | P0A799 | - |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, by gel filtration | Escherichia coli |
to homogeneity, by gel filtration | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PGK | - |
Escherichia coli |
PGK | - |
Saccharomyces cerevisiae |