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Literature summary for 2.7.2.3 extracted from

  • Young, T.A.; Skordalakes, E.; Marqusee, S.
    Comparison of proteolytic susceptibility in phosphoglycerate kinases from yeast and E. coli: modulation of conformational ensembles without altering structure or stability (2007), J. Mol. Biol., 368, 1438-1447.
    View publication on PubMed

Application

Application Comment Organism
additional information not resistant to proteolytic cleavage, both domains of the yeast protein fold in isolation into stable structures, extremely similar tertiary structures and global stabilities as compared to the homologous Escherichia coli PGK, but significant differences in proteolysis Saccharomyces cerevisiae
additional information resistant to proteolytic cleavage, the C-domain of PGK cannot be expressed or fold independently of the N-domain, extremely similar tertiary structures and global stabilities as compared to the homologous Saccharomyces cerevisiae PGK, but significant differences in proteolysis Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET27b and expressed in Escherichia coli BL21 (DE3)pLysS cells Saccharomyces cerevisiae
into vector pET27b and expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
in the presence of 1 mM AMP-PNP and 1 mM MgCl2, by the vapor diffusion method, solved by molecular replacement to 2.4 A resolution Escherichia coli
vapor diffusion method Escherichia coli

General Stability

General Stability Organism
phosphoglycerate kinase is resistant to proteolytic cleavage. ecPGK can only be digested through its globally unfolded state, suggesting that cooperativity between the two domains results in a more rigid protein that has low susceptibility to proteolysis Escherichia coli
the extrapolated unfolding rate of Escherichia coli PGK is 100000 slower than that of the yeast homolog Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli P0A799
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, by gel filtration Escherichia coli
to homogeneity, by gel filtration Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
PGK
-
Escherichia coli
PGK
-
Saccharomyces cerevisiae