Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | in bloodstream forms, after XRNA depletion, abundance of PGKB mRNA increases to about one-third of the PGKC mRNA abundance, with a corresponding decrease in the apparent degradation rate. The degradation of PGKC mRNA is not affected. In procyclic forms, PGKB mRNA is unaffected by XRNA depletion, PGKC mRNA is undetectable, but after XRNA depletion, PGKC mRNA is detectable | Trypanosoma brucei |
Application | Comment | Organism |
---|---|---|
additional information | PGKB mRNA and PGKB protein are much more abundant in the procyclic life-cycle stage than in bloodstream forms, whereas PGKC mRNA and PGKC protein are specific to bloodstream forms. PGKC 3'-UTR exerts strong regulatory effects, they are exerted at the levels of both mRNA stability and translation. PGKC3'-UTR contains some elements which promote active degradation in procyclic forms, but it also is responsible for an active stabilisation process in bloodstream forms | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinomycin D | blocks transcription | Trypanosoma brucei | |
additional information | addition of sinefungin prior to actinomycin D is the best protocol for complete and rapid inhibition of mRNA synthesis, thus in bloodstream forms, inhibition of both trans splicing and transcription results in immediate exponential decay of PGKC mRNA with a half-life of 46 min. Inhibition of transcription alone gives non-exponential kinetics and inhibition of splicing alone results in a longer apparent half-life | Trypanosoma brucei | |
sinefungin | blocks splicing | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | PGKB | Trypanosoma brucei | 5829 | - |
glycosome | - |
Trypanosoma brucei | 20015 | - |
glycosome | PGKC | Trypanosoma brucei | 20015 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma brucei | PGKC 3'-UTR exerts strong regulatory effects in Trypanosoma brucei. The regulatory effects are exerted at the levels of both mRNA stability and translation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bloodstream form | PGKB and PGKC genes-encoding phosphoglycerate kinase are co-transcribed as part of a polycistronic RNA. PGKC mRNA and glycosomal PGKC protein are specific to bloodstream forms | Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PGKC 3'-UTR exerts strong regulatory effects in Trypanosoma brucei. The regulatory effects are exerted at the levels of both mRNA stability and translation | Trypanosoma brucei | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGKB | - |
Trypanosoma brucei |
PGKC | - |
Trypanosoma brucei |