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Literature summary for 2.7.2.3 extracted from

  • Szabo, J.; Varga, A.; Flachner, B.; Konarev, P.V.; Svergun, D.I.; Zavodszky, P.; Vas, M.
    Communication between the nucleotide site and the main molecular hinge of 3-phosphoglycerate kinase (2008), Biochemistry, 47, 6735-6744.
    View publication on PubMed
Search for more literature for 2.7.2.3

Protein Variants

Protein Variants Comment Organism
E343A kcat for reaction with ATP + 3-phosphoglycerate is 58fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 31fold lower than wild-type value Homo sapiens
K215A kcat for reaction with ATP + 3-phosphoglycerate is 1735fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 3376fold lower than wild-type value Homo sapiens
K219A kcat for reaction with ATP + 3-phosphoglycerate is 925fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 502fold lower than wild-type value Homo sapiens
N336A kcat for reaction with ATP + 3-phosphoglycerate is 139fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 113fold lower than wild-type value Homo sapiens
R38A kcat for reaction with ATP + 3-phosphoglycerate is 1851fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 439fold lower than wild-type value Homo sapiens
T375A kcat for reaction with ATP + 3-phosphoglycerate is 6.7fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 3.9fold lower than wild-type value Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 3-phospho-D-glyceroyl phosphate mutant enzyme E343A Homo sapiens
0.0022
-
 3-phospho-D-glyceroyl phosphate mutant enzyme N336A Homo sapiens
0.003
-
 3-phospho-D-glyceroyl phosphate mutant enzyme T375A Homo sapiens
0.0038
-
 3-phospho-D-glyceroyl phosphate mutant enzyme K125A Homo sapiens
0.005
-
 3-phospho-D-glyceroyl phosphate wild-type enzyme Homo sapiens
0.0077
-
 3-phospho-D-glyceroyl phosphate mutant enzyme k219A Homo sapiens
0.1
-
 3-phospho-D-glycerate wild-type enzyme Homo sapiens
0.1
-
 ADP mutant enzyme R38A Homo sapiens
0.11
-
 ATP wild-type enzyme Homo sapiens
0.12
-
 ADP wild-type enzyme Homo sapiens
0.15
-
 ADP mutant enzyme K125A Homo sapiens
0.29
-
 3-phospho-D-glyceroyl phosphate mutant enzyme R38A Homo sapiens
0.31
-
 3-phospho-D-glycerate mutant enzyme E343A Homo sapiens
0.41
-
 3-phospho-D-glycerate mutant enzyme K125A Homo sapiens
0.42
-
 ADP mutant enzyme N336A Homo sapiens
0.54
-
 3-phospho-D-glycerate mutant enzyme N336A Homo sapiens
0.63
-
 3-phospho-D-glycerate mutant enzyme T375A Homo sapiens
0.91
-
 ADP mutant enzyme E343A Homo sapiens
0.96
-
 ATP mutant enzyme R38A Homo sapiens
1.04
-
 ATP mutant enzyme T375A Homo sapiens
1.12
-
 ADP mutant enzyme T375A Homo sapiens
1.34
-
 ATP mutant enzyme N336A Homo sapiens
1.42
-
 ADP mutant enzyme k219A Homo sapiens
1.69
-
 ATP mutant enzyme E343A Homo sapiens
1.72
-
 3-phospho-D-glycerate mutant enzyme k219A Homo sapiens
2.47
-
 ATP mutant enzyme K125A Homo sapiens
4.43
-
 ATP mutant enzyme k219A Homo sapiens
4.5
-
 3-phospho-D-glycerate mutant enzyme R38A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00558
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + 3-phospho-D-glyceroyl phosphate catalytic residue R38, which also binds the substrate 3-phosphoglycerate, is essential in inducing domain closure. Similarly, residues K219, N336, and E343 which interact with the nucleotide substrates are involved in the process of domain closure. The other catalytic residue, K215, covers a large distance during catalysis but has no direct role in domain closure Homo sapiens ATP + 3-phospho-D-glycerate
-
 ?

Synonyms

Synonyms Comment Organism
3-phosphoglycerate kinase
-
 Homo sapiens
hPGK
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 ATP mutant enzyme R38A Homo sapiens
0.45
-
 3-phospho-D-glycerate mutant enzyme R38A Homo sapiens
0.48
-
 ATP mutant enzyme K125A Homo sapiens
0.48
-
 3-phospho-D-glycerate mutant enzyme K125A Homo sapiens
0.78
-
 ADP mutant enzyme K125A Homo sapiens
0.78
-
 3-phospho-D-glyceroyl phosphate mutant enzyme K125A Homo sapiens
0.9
-
 ATP mutant enzyme k219A Homo sapiens
0.9
-
 3-phospho-D-glycerate mutant enzyme k219A Homo sapiens
5.25
-
 ADP mutant enzyme k219A Homo sapiens
5.25
-
 3-phospho-D-glyceroyl phosphate mutant enzyme k219A Homo sapiens
6
-
 ATP mutant enzyme N336A Homo sapiens
6
-
 3-phospho-D-glycerate mutant enzyme N336A Homo sapiens
6
-
 ADP mutant enzyme R38A Homo sapiens
6
-
 3-phospho-D-glyceroyl phosphate mutant enzyme R38A Homo sapiens
14.3
-
 ATP mutant enzyme E343A Homo sapiens
14.3
-
 3-phospho-D-glycerate mutant enzyme E343A Homo sapiens
23.2
-
 3-phospho-D-glyceroyl phosphate mutant enzyme N336A Homo sapiens
23.3
-
 ADP mutant enzyme N336A Homo sapiens
84
-
 ADP mutant enzyme E343A Homo sapiens
84
-
 3-phospho-D-glyceroyl phosphate mutant enzyme E343A Homo sapiens
125
-
 ATP mutant enzyme T375A Homo sapiens
125
-
 3-phospho-D-glycerate mutant enzyme T375A Homo sapiens
675
-
 ADP mutant enzyme T375A Homo sapiens
675
-
 3-phospho-D-glyceroyl phosphate mutant enzyme T375A Homo sapiens
833
-
 ATP wild-type enzyme Homo sapiens
833
-
 3-phospho-D-glycerate wild-type enzyme Homo sapiens
2633
-
 ADP wild-type enzyme Homo sapiens
2633
-
 3-phospho-D-glyceroyl phosphate wild-type enzyme Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.09
-
 diphosphate mutant enzyme N336A Homo sapiens
0.13
-
 diphosphate mutant enzyme R38A Homo sapiens
0.14
-
 diphosphate mutant enzyme E343A Homo sapiens
0.22
-
 diphosphate wild-type enzyme Homo sapiens
0.51
-
 diphosphate mutant enzyme K125A Homo sapiens
0.52
-
 diphosphate mutant enzyme k219A Homo sapiens
1.9
-
 diphosphate mutant enzyme T375A Homo sapiens