Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli at optimal growth temperatures (37°C) yields a product which shows a tight association with a 28000 Da protein and exhibits low thermal stability suggesting a misfolding of the protein. As proved by N-terminal amino acid sequence analysis, the 28000 Da protein represents a 226-amino acid C-terminal fragment of the 3-phosphoglycerate kinase. Mutagenesis experiments confirm the assumption that the fragment originates by an internal translation initiation | Pyrococcus woesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus woesei | P61884 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PwPGK | - |
Pyrococcus woesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
the enzyme from the original organism is completely resistant to heat inactivation for 35 min. Expression in Escherichia coli at optimal growth temperatures (37°C) yields a product which shows a tight association with a 28000 Da protein and exhibits low thermal stability (about 80% loss of activity after 5 min) suggesting a misfolding of the protein. As proved by N-terminal amino acid sequence analysis, the 28000 Da protein represents a 226-amino acid C-terminal fragment of the 3-phosphoglycerate kinase. Mutagenesis experiments confirm the assumption that the fragment originates by an internal translation initiation | Pyrococcus woesei |