Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-lysine | slightly stimulatory | Pseudomonas fluorescens | |
L-methionine | slightly stimulatory | Pseudomonas fluorescens | |
L-threonine | slight activation at low concentration | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | concerted feedback inhibition with L-threonine | Pseudomonas fluorescens | |
L-threonine | above 10 mM | Pseudomonas fluorescens | |
L-Threonine methyl ester | in combination with either L-lysine or L-methionine | Pseudomonas fluorescens | |
methionine | concerted feedback inhibition with L-threonine | Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | addition of 200 mM increases the reaction rate by about 40% | Pseudomonas fluorescens | |
Mn2+ | - |
Pseudomonas fluorescens | |
additional information | Li+ and Na+ ions have no effect, Zn2+, Ca2+ and Co2+ cannot replace Mg2+ or Mn2+ | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
3 * 43000, SDS-PAGE | Pseudomonas fluorescens |
133000 | - |
sedimentation velocity centrifugation | Pseudomonas fluorescens |
137000 | - |
gel filtration | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Pseudomonas fluorescens | first step common to the biosynthesis of L-lysine, L-threonine, isoleucine and methionine | ADP + 4-phospho-L-aspartate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
wild-type strain P-14 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.16 | - |
- |
Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Pseudomonas fluorescens | ADP + 4-phospho-L-aspartate | - |
r | |
ATP + L-aspartate | first step common to the biosynthesis of L-lysine, L-threonine, isoleucine and methionine | Pseudomonas fluorescens | ADP + 4-phospho-L-aspartate | - |
r | |
additional information | absolute requirement for ATP, no other nucleoside phosphates serve as phosphate donor, analogs of aspartate such as L-glutamate, DL-alpha-methyl aspartate, N-acetyl aspartate and D-aspartate are inactive as acceptors | Pseudomonas fluorescens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 43000, SDS-PAGE | Pseudomonas fluorescens |