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Literature summary for 2.7.2.4 extracted from

  • Wong, K.F.; Dennis, D.T.
    Aspartokinase from wheat germ (1973), Plant Physiol., 51, 322-326.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.4

General Stability

General Stability Organism
L-lysine and L-threonine partially prevents from heat inactivation Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
DL-2,6-Diaminoheptanoate
-
 Triticum aestivum
DL-meso-diaminopimelic acid
-
 Triticum aestivum
L-lysine
-
 Triticum aestivum
L-threonine concerted inhibition together with L-lysine Triticum aestivum
additional information no inhibition with L-homoserine, L-methionine and L-isoleucine Triticum aestivum
N-epsilon-formyl-L-lysine
-
 Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for MgCl2 3.3 mM Triticum aestivum
1.2
-
 ATP pH 7.8, 27°C Triticum aestivum
1.3
-
 ATP pH 7.8, 27°C Triticum aestivum
16.7
-
 L-aspartate pH 7.8, 27°C Triticum aestivum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Triticum aestivum
Mn2+
-
 Triticum aestivum
additional information Ca2+ shows a trace of activity, Zn2+ is inactive Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate Triticum aestivum
-
 ADP + 4-phospho-L-aspartate
-
 r

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
 wheat
-

Purification (Commentary)

Purification (Comment) Organism
partially Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
germ
-
 Triticum aestivum
-

Storage Stability

Storage Stability Organism
-15°C, dissolved in 0.05 M TES buffer containing 30% glycerol, pH 8.0, activity remains unchanged after 2.5 months Triticum aestivum
4°C, dissolved in 0.05 M TES buffer containing 30% glycerol, pH 8.0, activity remains unchanged after 6 days Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
 Triticum aestivum ADP + 4-phospho-L-aspartate
-
 r
GTP + L-aspartate
-
 Triticum aestivum GDP + 4-phospho-L-aspartate
-
 r
additional information no reaction observed with ITP, CTP or UTP Triticum aestivum ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 loses 70% of its original activity after heating for 15 min Triticum aestivum