Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.2.4 extracted from

  • Kobashi, N.; Nishiyama, M.; Tanokura, M.
    Kinetic and mutation analyses of aspartate kinase from Thermus flavus (1999), J. Biosci. Bioeng., 87, 739-745.
    View publication on PubMed
Search for more literature for 2.7.2.4

Activating Compound

Activating Compound Comment Organism Structure
L-threonine 0.01 mM, mutant G135A, activity 110%, mutant R150A, activity 115% Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
2 subunits, alpha and beta, encoded by an inframe overlapping gene, askAB genes cloned and expressed in Escherichia coli GT3 Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
A157L site-directed mutagenesis Thermus thermophilus
A189L site-directed mutagenesis Thermus thermophilus
A23L site-directed mutagenesis Thermus thermophilus
A42S site-directed mutagenesis Thermus thermophilus
D154A site-directed mutagenesis Thermus thermophilus
D154N site-directed mutagenesis Thermus thermophilus
D174A site-directed mutagenesis Thermus thermophilus
D182A site-directed mutagenesis Thermus thermophilus
E202A site-directed mutagenesis Thermus thermophilus
F136A site-directed mutagenesis Thermus thermophilus
G10A site-directed mutagenesis Thermus thermophilus
G135A site-directed mutagenesis Thermus thermophilus
G149A site-directed mutagenesis Thermus thermophilus
G152A site-directed mutagenesis Thermus thermophilus
G177A site-directed mutagenesis Thermus thermophilus
G73A site-directed mutagenesis Thermus thermophilus
I171A site-directed mutagenesis Thermus thermophilus
L148A site-directed mutagenesis Thermus thermophilus
P183A site-directed mutagenesis Thermus thermophilus
R150A site-directed mutagenesis Thermus thermophilus
S12A site-directed mutagenesis Thermus thermophilus
S153A site-directed mutagenesis Thermus thermophilus
S41A site-directed mutagenesis Thermus thermophilus
T238A site-directed mutagenesis Thermus thermophilus
T47A site-directed mutagenesis Thermus thermophilus
V39A site-directed mutagenesis Thermus thermophilus
Y8A site-directed mutagenesis Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 L-aspartate pH 7.5, 60°C, mutant A157L Thermus thermophilus
0.27
-
 L-aspartate pH 7.5, 60°C, mutant E202A Thermus thermophilus
0.27
-
 ATP pH 7.5, 60°C, mutant G177A Thermus thermophilus
0.29
-
 ATP pH 7.5, 60°C, mutant G149A Thermus thermophilus
0.32
-
 L-aspartate pH 7.5, 60°C, mutant Y8A, S12A Thermus thermophilus
0.33
-
 L-aspartate pH 7.5, 60°C, mutant V39A Thermus thermophilus
0.34
-
 L-aspartate pH 7.5, 60°C, mutant G177A Thermus thermophilus
0.36
-
 L-aspartate pH 7.5, 60°C, mutant G149A, G177A Thermus thermophilus
0.37
-
 L-aspartate pH 7.5, 60°C, mutant I171A Thermus thermophilus
0.39
-
 L-aspartate pH 7.5, 60°C, mutant T238A Thermus thermophilus
0.41
-
 ATP pH 7.5, 60°C, mutant G149A Thermus thermophilus
0.41
-
 L-aspartate pH 7.5, 60°C, mutant S41A Thermus thermophilus
0.42
-
 L-aspartate pH 7.5, 60°C, mutant G152A, P183A Thermus thermophilus
0.43
-
 ATP pH 7.5, 60°C, mutant D174A Thermus thermophilus
0.44
-
 ATP pH 7.5, 60°C, mutant S153A Thermus thermophilus
0.45
-
 ATP pH 7.5, 60°C, mutant A157L Thermus thermophilus
0.45
-
 L-aspartate pH 7.5, 60°C, mutant A189L Thermus thermophilus
0.46
-
 ATP pH 7.5, 60°C, mutant G150A Thermus thermophilus
0.47
-
 L-aspartate pH 7.5, 60°C, mutant S153A Thermus thermophilus
0.5
-
 ATP pH 7.5, 60°C, wild-type and mutant G152A Thermus thermophilus
0.52
-
 ATP pH 7.5, 60°C, mutant S12A, G135A Thermus thermophilus
0.53
-
 L-aspartate pH 7.5, 60°C, mutant D174A Thermus thermophilus
0.56
-
 ATP pH 7.5, 60°C, mutant T238A Thermus thermophilus
0.57
-
 ATP pH 7.5, 60°C, mutant F136A Thermus thermophilus
0.6
-
 ATP pH 7.5, 60°C, mutant I171A Thermus thermophilus
0.6
-
 L-aspartate pH 7.5, 60°C, mutant A42S Thermus thermophilus
0.61
-
 L-aspartate pH 7.5, 60°C, mutant G73A Thermus thermophilus
0.63
-
 ATP pH 7.5, 60°C, mutant D154N Thermus thermophilus
0.65
-
 L-aspartate pH 7.5, 60°C, mutant A23L Thermus thermophilus
0.72
-
 ATP pH 7.5, 60°C, mutant A23L Thermus thermophilus
0.86
-
 ATP pH 7.5, 60°C, mutant Y8A Thermus thermophilus
0.89
-
 ATP pH 7.5, 60°C, mutant V39A Thermus thermophilus
0.97
-
 ATP pH 7.5, 60°C, mutant L148A Thermus thermophilus
0.98
-
 ATP pH 7.5, 60°C, mutant A189L Thermus thermophilus
1.02
-
 ATP pH 7.5, 60°C, mutant A42S Thermus thermophilus
1.05
-
 ATP pH 7.5, 60°C, mutant D154A Thermus thermophilus
1.2
-
 L-aspartate pH 7.5, 60°C, mutant P183A Thermus thermophilus
1.23
-
 L-aspartate pH 7.5, 60°C, mutant R150A, D154A Thermus thermophilus
1.28
-
 ATP pH 7.5, 60°C, mutant G73A Thermus thermophilus
1.31
-
 L-aspartate pH 7.5, 60°C, D182A Thermus thermophilus
1.34
-
 L-aspartate pH 7.5, 60°C, mutant G10A, G135A Thermus thermophilus
1.39
-
 ATP pH 7.5, 60°C, mutant S41A Thermus thermophilus
1.9
-
 ATP pH 7.5, 60°C, mutant P183A Thermus thermophilus
2.1
-
 L-aspartate pH 7.5, 60°C, mutant L148A Thermus thermophilus
2.1
-
 ATP pH 7.5, 60°C, mutant T47A Thermus thermophilus
2.45
-
 ATP pH 7.5, 60°C, mutant D182A Thermus thermophilus
3.57
-
 ATP pH 7.5, 60°C, mutant G10A Thermus thermophilus
5.29
-
 L-aspartate pH 7.5, 60°C, mutant F136A Thermus thermophilus
19.5
-
 L-aspartate pH 7.5, 60°C, mutant T47A Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 1 * 17000 + 1 * 43000, SDS-PAGE Thermus thermophilus
43000
-
 1 * 17000 + 1 * 43000, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate Thermus thermophilus first step of the branched biosynthetic pathway for lysine, threonine, isoleucine and methionine ADP + 4-phospho-L-aspartate
-
 r

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in E. coli Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
 Thermus thermophilus

Storage Stability

Storage Stability Organism
4°C, purified enzyme stable for at least 2 weeks in gel filtration buffer Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
 Thermus thermophilus ADP + 4-phospho-L-aspartate
-
 r
ATP + L-aspartate first step of the branched biosynthetic pathway for lysine, threonine, isoleucine and methionine Thermus thermophilus ADP + 4-phospho-L-aspartate
-
 r

Subunits

Subunits Comment Organism
heterodimer 1 * 17000 + 1 * 43000, SDS-PAGE Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 L-aspartate pH 7.5, 60°C, mutant S41A Thermus thermophilus
0.967
-
 L-aspartate pH 7.5, 60°C, mutant G135A Thermus thermophilus
1.83
-
 L-aspartate pH 7.5, 60°C, mutant R150A, D154A Thermus thermophilus
2.17
-
 L-aspartate pH 7.5, 60°C, mutant D154N, D182A Thermus thermophilus
2.83
-
 L-aspartate pH 7.5, 60°C, mutant G10A Thermus thermophilus
3
-
 L-aspartate pH 7.5, 60°C, mutant D174A Thermus thermophilus
4
-
 L-aspartate pH 7.5, 60°C, mutant E202A Thermus thermophilus
4.33
-
 L-aspartate pH 7.5, 60°C, mutant G152A, P183A Thermus thermophilus
4.67
-
 L-aspartate pH 7.5, 60°C, mutant A157L Thermus thermophilus
6
-
 L-aspartate pH 7.5, 60°C, mutant A189L Thermus thermophilus
6.67
-
 L-aspartate pH 7.5, 60°C, mutant T47A Thermus thermophilus
7.33
-
 L-aspartate pH 7.5, 60°C, mutant T238A Thermus thermophilus
10.8
-
 L-aspartate pH 7.5, 60°C, mutant I171A Thermus thermophilus
12.3
-
 L-aspartate pH 7.5, 60°C, mutant S153A Thermus thermophilus
13.3
-
 L-aspartate pH 7.5, 60°C, mutant G149A, G177A Thermus thermophilus
13.8
-
 L-aspartate pH 7.5, 60°C, mutant Y8A Thermus thermophilus
16.7
-
 L-aspartate pH 7.5, 60°C, mutant F136A Thermus thermophilus
23.2
-
 L-aspartate pH 7.5, 60°C, mutant A42S Thermus thermophilus
23.3
-
 L-aspartate pH 7.5, 60°C, mutant L148A Thermus thermophilus
24.3
-
 L-aspartate pH 7.5, 60°C, mutant A23L Thermus thermophilus
24.7
-
 L-aspartate pH 7.5, 60°C, mutant S12A Thermus thermophilus
25
-
 L-aspartate pH 7.5, 60°C, wild-type Thermus thermophilus
31.7
-
 L-aspartate pH 7.5, 60°C, mutant G73A Thermus thermophilus
32
-
 L-aspartate pH 7.5, 60°C, mutant V39A Thermus thermophilus