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Literature summary for 2.7.2.4 extracted from

  • Kato, C.; Kurihara, T.; Kobashi, N.; Yamane, H.; Nishiyama, M.
    Conversion of feedback regulation in aspartate kinase by domain exchange (2004), Biochem. Biophys. Res. Commun., 316, 802-808.
    View publication on PubMed
Search for more literature for 2.7.2.4

Activating Compound

Activating Compound Comment Organism Structure
L-threonine enhances activity up to 140% at 0.2 mM Bacillus subtilis
L-threonine enhances activity up to 140% at 0.2 mM Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis
expression in Escherichia coli Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
L-lysine no inhibition is observed; the truncated protein is no longer inhibited by lysine, even at 10 mM, wild type protein is inhibited by lysine Bacillus subtilis
L-lysine no inhibition is observed Thermus thermophilus
L-threonine almost insensitive to threonine Bacillus subtilis
L-threonine
-
 Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.9
-
 L-aspartate
-
 Bacillus subtilis
3.9
-
 L-aspartate
-
 Thermus thermophilus
4.5
-
 ATP
-
 Bacillus subtilis
4.5
-
 ATP
-
 Thermus thermophilus
6.5
-
 ATP wild type Bacillus subtilis
6.9
-
 ATP
-
 Bacillus subtilis
6.9
-
 ATP
-
 Thermus thermophilus
10
-
 L-aspartate wild type Bacillus subtilis
10.6
-
 L-aspartate
-
 Bacillus subtilis
10.6
-
 L-aspartate
-
 Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 beta subunit, calculated from nucleotide sequence Thermus thermophilus
18000
-
 alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence Bacillus subtilis
18500
-
 beta subunit, calculated from nucleotide sequence Bacillus subtilis
42700
-
 alpha subunit, calculated from nucleotide sequence Bacillus subtilis
42700
-
 alpha subunit, calculated from nucleotide sequence Thermus thermophilus
43200
-
 alpha subunit, calculated from nucleotide sequence Thermus thermophilus
43700
-
 alpha subunit, calculated from nucleotide sequence Bacillus subtilis
43900
-
 alpha subunit, calculated from nucleotide sequence Bacillus subtilis
43900
-
 alpha subunit, calculated from nucleotide sequence Thermus thermophilus
60700
-
 alphabeta, calculated from nucleotide sequence Bacillus subtilis
60700
-
 alphabeta, calculated from nucleotide sequence Thermus thermophilus
61200
-
 alphabeta, calculated from nucleotide sequence Thermus thermophilus
62200
-
 alphabeta, calculated from nucleotide sequence Bacillus subtilis
64200
-
 alpha2, gel filtration Bacillus subtilis
64200
-
 alpha2, gel filtration Thermus thermophilus
87800
-
 alpha2, calculated from nucleotide sequence Bacillus subtilis
87800
-
 alpha2, calculated from nucleotide sequence Thermus thermophilus
90300
-
 alpha2, beta2, gel filtration Thermus thermophilus
121400
-
 alpha2, beta2, calculated from nucleotide sequence Bacillus subtilis
121400
-
 alpha2, beta2, calculated from nucleotide sequence Thermus thermophilus
122400
-
 alpha2, beta2, calculated from nucleotide sequence Thermus thermophilus
124400
-
 alpha2, beta2, calculated from nucleotide sequence Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate Bacillus subtilis
-
 ADP + phospho-L-aspartate
-
 ?
ATP + L-aspartate Thermus thermophilus
-
 ADP + phospho-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis
-
 chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus
-
Bacillus subtilis
-
 chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus, and the beta subunit from Thermus flavus
-
Thermus thermophilus
-
-
-
Thermus thermophilus
-
 chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus
-
Thermus thermophilus
-
 chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus, and the beta subunit from Thermus flavus
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.38
-
 truncated protein, which contains only the N-terminal region Bacillus subtilis
5.9
-
 wild type Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
 Bacillus subtilis ADP + phospho-L-aspartate
-
 ?
ATP + L-aspartate
-
 Thermus thermophilus ADP + phospho-L-aspartate
-
 ?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 43900, gel filtration, calculated from nucleotide sequence Bacillus subtilis
dimer alpha2, 2 * 43900, gel filtration, calculated from nucleotide sequence Thermus thermophilus
tetramer alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence Bacillus subtilis
tetramer alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence Thermus thermophilus
tetramer alpha2, beta2, 2 * 43200 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence Thermus thermophilus
tetramer alpha2, beta2, 2 * 43700 + 2 * 18500, calculated from nucleotide sequence Bacillus subtilis

Synonyms

Synonyms Comment Organism
AK
-
 Thermus thermophilus
AK II
-
 Bacillus subtilis
aspartate kinase
-
 Bacillus subtilis
aspartate kinase
-
 Thermus thermophilus
BT
-
 Bacillus subtilis
BT
-
 Thermus thermophilus
BTT
-
 Bacillus subtilis
BTT
-
 Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 AK II loses activity very rapidly with a half-life of 31.5 s Bacillus subtilis
70
-
 AK retains the full activity after 1 h of heat treatment Thermus thermophilus
70
-
 loss of activity with a half-life of 45.7 s Bacillus subtilis
70
-
 loss of activity with a half-life of 45.7 s Thermus thermophilus
70
-
 no loss of activity even after 1 h of heat treatment Bacillus subtilis
70
-
 no loss of activity even after 1 h of heat treatment Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.87
-
 ATP
-
 Bacillus subtilis
11.87
-
 ATP
-
 Thermus thermophilus
15.25
-
 ATP
-
 Bacillus subtilis
15.25
-
 ATP
-
 Thermus thermophilus
15.87
-
 ATP wild type Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11
-
 L-threonine
-
 Bacillus subtilis
1.11
-
 L-threonine
-
 Thermus thermophilus
1.24
-
 L-threonine
-
 Thermus thermophilus
1.28
-
 L-lysine
-
 Bacillus subtilis