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Literature summary for 2.7.2.7 extracted from

  • Twarog, R.; Wolfe, R.S.
    Enzymic phosphorylation of butyrate (1962), J. Biol. Chem., 237, 2474-2477.
    View publication on PubMed
Search for more literature for 2.7.2.7

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 10 mM, 47% inhibition Clostridium butyricum
PCMB 0.1 mM, 66% inhibition Clostridium butyricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 ATP pH 7.2, 30°C Clostridium butyricum
9
-
 Isobutanoate pH 7.2, 30°C Clostridium butyricum
20
-
 Butanoate pH 7.2, 30°C Clostridium butyricum
26
-
 propionate pH 7.2, 30°C Clostridium butyricum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight activation Clostridium butyricum
Fe2+ slight activation Clostridium butyricum
Mg2+ cation requirement is satisfied by Mn2+ or Mg2+ Clostridium butyricum
Mn2+ cation requirement is satisfied by Mn2+ or Mg2+. Km-value for Mn2+ is 27 mM Clostridium butyricum
Zn2+ slight activation Clostridium butyricum

Organism

Organism UniProt Comment Textmining
Clostridium butyricum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate poor substrate Clostridium butyricum ADP + acetyl phosphate
-
 ?
ATP + butanoate
-
 Clostridium butyricum ADP + butanoyl phosphate
-
 r
ATP + isobutanoate 50% of the activity with butanoate Clostridium butyricum ADP + isobutanoyl phosphate
-
 ?
ATP + propionate as active as butanoate Clostridium butyricum ADP + propanoyl phosphate
-
 ?
ATP + valerate 12% of the activity with butanoate Clostridium butyricum ADP + pentanoyl phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Clostridium butyricum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.8 pH 6.5: about 60% of maximal activity pH 7.8: about 65% of maximal activity Clostridium butyricum