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Literature summary for 2.7.2.7 extracted from
- Butyrate kinase from Clostridium acetobutylicum (1987), J. Biol. Chem., 262, 617-621.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | presence of a reducing agent is required | Clostridium acetobutylicum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 20 mM; 49% inhibition | Clostridium acetobutylicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | - |
Butanoate | pH 7.6 | Clostridium acetobutylicum | |
| 14 | - |
valerate | pH 7.6 | Clostridium acetobutylicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Km: 2.0 mM | Clostridium acetobutylicum | |
| Mn2+ | Km: 4.0 mM | Clostridium acetobutylicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
2 * 39000, SDS-PAGE | Clostridium acetobutylicum |
| 85000 | - |
non-denaturing PAGE | Clostridium acetobutylicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 402 | - |
- |
Clostridium acetobutylicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 25 mM Tris-HCl, pH 7.6, 1 mM DTT, stable for several months | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | 6% of the activity with butanoate | Clostridium acetobutylicum | ADP + acetyl phosphate | - |
? | |
| ATP + butanoate | - |
Clostridium acetobutylicum | ADP + butanoyl phosphate | - |
r | |
| ATP + isobutanoate | 54% of the activity with butanoate | Clostridium acetobutylicum | ADP + isobutanoyl phosphate | - |
? | |
| ATP + isovalerate | 32% of the activity with butanoate | Clostridium acetobutylicum | ADP + isopentanoyl phosphate | - |
? | |
| ATP + propionate | 43% of the activity with butanoate | Clostridium acetobutylicum | ADP + propanoyl phosphate | - |
? | |
| ATP + valerate | 89% of the activity with butanoate | Clostridium acetobutylicum | ADP + pentanoyl phosphate | - |
? | |
| ATP + vinyl acetate | 23% of the activity with butanoate | Clostridium acetobutylicum | ADP + vinylacetyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 39000, SDS-PAGE | Clostridium acetobutylicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
20 min, 70% loss of activity in absence of 100 mM butanoate, complete protection against inactivation in presence of mM potassium butanoate | Clostridium acetobutylicum |
| 60 | - |
1 min, complete loss of activity in absence of butanoate | Clostridium acetobutylicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
formation of butanoyl phosphate | Clostridium acetobutylicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6: about 25% of maximal activity, pH 10.0: about 50% of maximal activity | Clostridium acetobutylicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | very stable | Clostridium acetobutylicum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Clostridium acetobutylicum | isoelectric focusing | 5.9 | 5.6 |
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