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Literature summary for 2.7.2.8 extracted from
- Mechanism of arginine biosynthesis in Chlamydomonas reinhardti. II. Purification and properties of N-acetylglutamate 5-phosphotransferase, the allosteric enzyme of the pathway (1967), Biochim. Biophys. Acta, 136, 6-18.
General Stability
| General Stability | Organism |
|---|---|
| L-arginine protects against inactivating effects of high concentrations of urea | Chlamydomonas reinhardtii |
| L-arginine protects against the inactivating effects of heat | Chlamydomonas reinhardtii |
| urea, 4.0 M, complete loss of activity after 120 min | Chlamydomonas reinhardtii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arginine | pH-optimum for inhibition is 7.5 | Chlamydomonas reinhardtii |  |
| L-canavanine | - |
Chlamydomonas reinhardtii | |
| L-citrulline | - |
Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
ATP | pH 5.5, 37°C | Chlamydomonas reinhardtii | |
| 15 | - |
N-acetyl-L-glutamate | pH 5.5, 37°C | Chlamydomonas reinhardtii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Mg2+ or Co2+ required | Chlamydomonas reinhardtii | |
| Mg2+ | Mg2+ or Co2+ required | Chlamydomonas reinhardtii | |
| Mn2+ | less effective than Mg2+ and Co2+ in activation | Chlamydomonas reinhardtii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-L-glutamate | Chlamydomonas reinhardtii | key enzyme in regulation of arginine biosynthesis | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-L-glutamate | - |
Chlamydomonas reinhardtii | ADP + N-acetyl-L-glutamate 5-phosphate | in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate | ? | |
| ATP + N-acetyl-L-glutamate | key enzyme in regulation of arginine biosynthesis | Chlamydomonas reinhardtii | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Chlamydomonas reinhardtii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 64 | - |
10 min, 75% loss of activity without stabilizer, about 40% loss of activity with 75 mM N-acetyl-L-glutamate, 10 min, 10% loss of activity with 1 mM L-arginine | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Chlamydomonas reinhardtii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 7 | pH 4.6: about 75% of maximum activity, pH 7: about 60% of maximum activity | Chlamydomonas reinhardtii |
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