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Literature summary for 2.7.2.8 extracted from

  • Denes, G.
    N-Acetylglutamate-5-phosphotransferase (1973), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 9, 511-520.
No PubMed abstract available
Search for more literature for 2.7.2.8

General Stability

General Stability Organism
L-arginine protects against the inactivating effects of heat Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
arginine allosteric enzyme which is inhibited by arginine Chlamydomonas reinhardtii
arginine enzyme has no allosteric properties and its activity is influenced neither by arginine nor by any of the intermediates of the arginine biosynthetic pathway Escherichia coli
MgCl2 inhibition above 20 mM Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 ATP
-
 Escherichia coli
3
-
 ATP pH 8.0, 37°C, allosteric Chlamydomonas reinhardtii
4
-
 N-acetyl-L-glutamate pH 8.0, 37°C, allosteric Chlamydomonas reinhardtii
6
-
 N-acetyl-L-glutamate pH 5.5, 37°C Escherichia coli
15
-
 N-acetyl-L-glutamate
-
 Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Mg2+ or Co2+ required Chlamydomonas reinhardtii
Mg2+
-
 Escherichia coli
Mg2+ required Chlamydomonas reinhardtii
Mg2+ Mg2+ or Co2+ required Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
400000
-
 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-L-glutamate Chlamydomonas reinhardtii key enzyme in regulation of arginine biosynthesis ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate Escherichia coli key enzyme in regulation of arginine biosynthesis ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Escherichia coli
-
 ATCC 25542
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chlamydomonas reinhardtii
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate highly specific for ATP and N-acetyl-L-glutamate Chlamydomonas reinhardtii ADP + N-acetyl-L-glutamate 5-phosphate in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate ?
ATP + N-acetyl-L-glutamate highly specific for ATP and N-acetyl-L-glutamate Escherichia coli ADP + N-acetyl-L-glutamate 5-phosphate in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate ?
ATP + N-acetyl-L-glutamate key enzyme in regulation of arginine biosynthesis Chlamydomonas reinhardtii ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate key enzyme in regulation of arginine biosynthesis Escherichia coli ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
additional information not: GTP Chlamydomonas reinhardtii ?
-
 ?
additional information not: GTP Escherichia coli ?
-
 ?
additional information not: N-benzoyl-L-glutamate, L-glutamate, D-glutamate Chlamydomonas reinhardtii ?
-
 ?
additional information not: N-benzoyl-L-glutamate, L-glutamate, D-glutamate Escherichia coli ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Chlamydomonas reinhardtii
6.8 7.8
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5
-
 Chlamydomonas reinhardtii