Protein Variants | Comment | Organism |
---|---|---|
E17A | site-directed mutagenesis, the mutant shows reduced Vmax, the mutation results in decreased affinity of NAGK for arginine | Pseudomonas aeruginosa |
E17D | site-directed mutagenesis, the mutant shows reduced Vmax, the mutation results in decreased affinity of NAGK for arginine | Pseudomonas aeruginosa |
E17Q | site-directed mutagenesis, the mutant shows reduced Vmax, the mutation results in decreased affinity of NAGK for arginine | Pseudomonas aeruginosa |
E284D | site-directed mutagenesis, the mutant shows reduced Vmax and altered Km for the substrates | Pseudomonas aeruginosa |
G290A | site-directed mutagenesis, the mutant shows reduced Vmax and altered Km for the substrates | Pseudomonas aeruginosa |
H271N | site-directed mutagenesis, the mutant shows reduced Vmax and altered Km for the substrates | Pseudomonas aeruginosa |
K213 | site-directed mutagenesis, the mutant shows reduced Vmax and altered Km for the substrates | Pseudomonas aeruginosa |
additional information | N-helix N-terminal deletions spanning 16 residues dissociate NAGK to active dimers, those of 20 residues decrease the apparent affinity for arginine, and complete N-helix deletion of 26 residues abolishes arginine inhibition, overview | Pseudomonas aeruginosa |
Q10A | site-directed mutagenesis, the mutant shows reduced Vmax and altered Km for the substrates | Pseudomonas aeruginosa |
R24E | site-directed mutagenesis, the mutant shows reduced Vmax , the mutation results in increased affinity of NAGK for arginine | Pseudomonas aeruginosa |
Y21A | site-directed mutagenesis, the mutant shows reduced Vmax and altered Km for the substrates | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-arginine | sigmoidal arginine inhibition kinetics, feedback inhibition, indentification of the N-terminal arginine site, mutational analysis, the mobile alphaH-beta16 loop of the arginine site is the modulatory signal receiver, overview | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of enzyme mutants, overview | Pseudomonas aeruginosa | |
3.1 | - |
ATP | wild-type enzyme | Pseudomonas aeruginosa | |
3.1 | - |
N-acetyl-L-glutamate | wild-type enzyme | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | Pseudomonas aeruginosa | NAGK catalyzes the second step of arginine biosynthesis. In Pseudomonas aeruginosa, this step is rate limiting, and feedback regulated and sigmoidally inhibited by arginine | ADP + N-acetyl-L-glutamyl 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | - |
Pseudomonas aeruginosa | ADP + N-acetyl-L-glutamyl 5-phosphate | - |
? | |
ATP + N-acetyl-L-glutamate | NAGK catalyzes the second step of arginine biosynthesis. In Pseudomonas aeruginosa, this step is rate limiting, and feedback regulated and sigmoidally inhibited by arginine | Pseudomonas aeruginosa | ADP + N-acetyl-L-glutamyl 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | with an extra N-terminal-linked helix interlinking three dimers, the hexameric architecture is not essential for arginine inhibition but is functionally essential for physiologically relevant arginine control of NAGK | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
N-acetylglutamate kinase | - |
Pseudomonas aeruginosa |
NagK | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas aeruginosa |