Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 10 mM, 100% activation of guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
Guanidine HCl | 5 mM, 20% activation of guanosine 5'-tetraphosphate synthesis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F488A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
P507A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
Q674A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
R375A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
R564A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
R621A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
S380A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
Y468A | 120-140% of wild-type polyphosphate, GTP, and guanosine 5'-tetraphosphate synthesis activity, 20-50% of wild-type ATP synthesis activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 10 mM, 66% inhibition of polyphosphate synthesis, 75% inhibition of GTP synthesis | Escherichia coli | |
GMP | competitive inhibition of polyphosphate 750 and GDP in guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
Guanidine HCl | 5 mM, 50% inhibition of polyphosphate synthesis | Escherichia coli | |
Polyphosphate | 65 residues, competitive inhibition of polyphosphate 750 and GDP in guanosine 5'-tetraphosphate synthesis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
Polyphosphate | pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
0.16 | - |
GDP | pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity of polyphosphate synthesis, ATP and GTP synthesis, and guanosine 5'-tetraphosphate synthesis at 5.0, 2.0, 1.0 and 0.2 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | - |
Escherichia coli | ADP + (phosphate)n+1 | polyphosphate chains of lenths of 700-800 residues | ? | |
GDP + (phosphate)n+1 | - |
Escherichia coli | GTP + (phosphate)n | - |
? | |
GDP + (phosphate)n+2 | - |
Escherichia coli | guanosine 5'-tetraphosphate + (phosphate)n | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPK1 | i.e. polyphosphate kinase 1 | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.482 | - |
GDP | pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
1.04 | - |
Polyphosphate | pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
GMP | pH 7.5, 37°C, competitive inhibition of GDP in guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
0.0012 | - |
polyphosphate 65 | pH 7.5, 37°C, competitive inhibition of GDP in guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
0.0014 | - |
GMP | pH 7.5, 37°C, competitive inhibition of polyphosphate 750 in guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
0.0076 | - |
polyphosphate 65 | pH 7.5, 37°C, competitive inhibition of polyphosphate 750 in guanosine 5'-tetraphosphate synthesis | Escherichia coli |