Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | optimal at 80 mM | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
genes NCgl0880 and NCgl2620 or pkk2A and pkk2B, expression of wild-type and mutant PKK2B isozymes in Escherichia coli strain Bl21(DE3), subcloning in Escherichia coli strain DH5alpha | Corynebacterium glutamicum |
overexpression in Escherichia coli | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ppk2A and ppk2b knockout organisms, ppk2b has PPK activity | Corynebacterium glutamicum |
additional information | deletion of ppk2B decreases PPK activity and cellular polyphosphate content, while overexpression of ppk2B increases both PPK activity and cellular polyphosphate content. Neither deletion nor overexpression of ppk2A changes specific activity of PPK or cellular polyphosphate content significantly. The ppk2B deletion mutant, which accumulated very little polyphopshate and grows like the wild-type under phosphate-sufficient conditions, shows a growth defect under phosphate-limiting conditions, phenotype, overview | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Corynebacterium glutamicum | |
GMP | - |
Corynebacterium glutamicum | |
IMP | - |
Corynebacterium glutamicum | |
additional information | no inhibition by UMP and CMP | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
GTP | - |
Corynebacterium glutamicum | |
0.14 | - |
GTP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum | |
0.17 | - |
ATP | - |
Corynebacterium glutamicum | |
0.17 | - |
ATP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum | |
1 | - |
ITP | - |
Corynebacterium glutamicum | |
1 | - |
ITP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum | |
15 | - |
(Phosphate)n | - |
Corynebacterium glutamicum | |
15 | - |
Polyphosphate | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Corynebacterium glutamicum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal at 10 mM | Corynebacterium glutamicum | |
Mg2+ | optimal at 10 mM, activates | Corynebacterium glutamicum | |
Mn2+ | optimal at 10 mM | Corynebacterium glutamicum | |
Mn2+ | optimal at 10 mM, activates highly | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
151400 | - |
gel filtration | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | Corynebacterium glutamicum | PPK2B forms polyphopshate with an average chain length of about 125 | ADP + (phosphate)n+1 | - |
r | |
ATP + (phosphate)n | Corynebacterium glutamicum MH20-22B | PPK2B forms polyphopshate with an average chain length of about 125 | ADP + (phosphate)n+1 | - |
r | |
additional information | Corynebacterium glutamicum | accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview | ? | - |
? | |
additional information | Corynebacterium glutamicum MH20-22B | accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
MH20-22B | - |
Corynebacterium glutamicum | - |
wild-type strain ATCC 13032 is auxotrophic for biotin, genes NCgl0880 and NCgl2620 encoding class II isozymes PPK2A and PPK2B, no class I polyphosphate kinase, PPK1, encoded by ppk1, in this bacterium | - |
Corynebacterium glutamicum MH20-22B | - |
MH20-22B | - |
Purification (Comment) | Organism |
---|---|
of the overexpessed protein | Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Corynebacterium glutamicum |
14 | - |
purified recombinant wild-type enzyme, polyphosphate synthesis with GTP | Corynebacterium glutamicum |
18 | - |
purified recombinant wild-type enzyme, polyphosphate synthesis with ITP | Corynebacterium glutamicum |
31 | - |
purified recombinant wild-type enzyme, polyphosphate synthesis with ATP | Corynebacterium glutamicum |
32 | - |
purified recombinant wild-type enzyme, polyphosphate dephosphorylation | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | - |
Corynebacterium glutamicum | ADP + (phosphate)n+1 | - |
r | |
ATP + (phosphate)n | PPK2B forms polyphopshate with an average chain length of about 125 | Corynebacterium glutamicum | ADP + (phosphate)n+1 | - |
r | |
ATP + (phosphate)n | PPK2B forms polyphopshate with an average chain length of about 125 and prefers the polyphosphate synthesis reaction direction | Corynebacterium glutamicum | ADP + (phosphate)n+1 | polyphosphate product chain length determination by NMR spectroscopy | r | |
ATP + (phosphate)n | - |
Corynebacterium glutamicum MH20-22B | ADP + (phosphate)n+1 | - |
r | |
ATP + (phosphate)n | PPK2B forms polyphopshate with an average chain length of about 125 | Corynebacterium glutamicum MH20-22B | ADP + (phosphate)n+1 | - |
r | |
ATP + (phosphate)n | PPK2B forms polyphopshate with an average chain length of about 125 and prefers the polyphosphate synthesis reaction direction | Corynebacterium glutamicum MH20-22B | ADP + (phosphate)n+1 | polyphosphate product chain length determination by NMR spectroscopy | r | |
ATP + ATP | polyP not required as primer | Corynebacterium glutamicum | ADP + (phosphate)n+1 | - |
r | |
GTP + (phosphate)n | - |
Corynebacterium glutamicum | GDP + (phosphate)n+1 | - |
r | |
GTP + (phosphate)n | lower activity compared to ATP | Corynebacterium glutamicum | GDP + (phosphate)n+1 | - |
r | |
ITP + (phosphate)n | - |
Corynebacterium glutamicum | IDP + (phosphate)n+1 | - |
r | |
ITP + (phosphate)n | lower activity compared to ATP | Corynebacterium glutamicum | IDP + (phosphate)n+1 | - |
r | |
additional information | accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview | Corynebacterium glutamicum | ? | - |
? | |
additional information | PKK2B also shows NDP kinase activity | Corynebacterium glutamicum | ? | - |
? | |
additional information | accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview | Corynebacterium glutamicum MH20-22B | ? | - |
? | |
additional information | PKK2B also shows NDP kinase activity | Corynebacterium glutamicum MH20-22B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 x 39000, SDS PAGE | Corynebacterium glutamicum |
tetramer | PPK2B is active as a homotetramer | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
ATP:polyphosphate phosphotransferase | - |
Corynebacterium glutamicum |
class II polyphosphate kinase | - |
Corynebacterium glutamicum |
kinase, polyphosphate (phosphorylating) | - |
Corynebacterium glutamicum |
polyphosphate kinase | - |
Corynebacterium glutamicum |
PPK | - |
Corynebacterium glutamicum |
PPK2 | - |
Corynebacterium glutamicum |
PPK2B | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
preincubation results in a loss of 50% of the activity | Corynebacterium glutamicum |
20 | 25 | 30 min, purified recombinant wild-type PKK2B, 50% remaining activity | Corynebacterium glutamicum |
25 | - |
preincubation results in a loss of 50% of the activity | Corynebacterium glutamicum |
45 | - |
preincubation results in a loss of 50% of the activity with substrate | Corynebacterium glutamicum |
45 | - |
preincubation results in an almost complete loss the activity without substrate | Corynebacterium glutamicum |
45 | - |
30 min, purified recombinant wild-type PKK2B, almost no remaining activity in absence of polyphosphate, but about 50% remaining activity in presence of 40 mM polyphosphate | Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
GTP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum | |
43 | - |
ITP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum | |
74 | - |
ATP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum | |
77 | - |
Polyphosphate | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
narrow optimum | Corynebacterium glutamicum |
7.2 | - |
in 50mM TEA | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Corynebacterium glutamicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
GMP | - |
Corynebacterium glutamicum | |
3.9 | - |
GMP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum | |
6.6 | - |
IMP | - |
Corynebacterium glutamicum | |
6.6 | - |
IMP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum | |
7.9 | - |
AMP | no inhibition by CMP and UMP | Corynebacterium glutamicum | |
7.9 | - |
AMP | pH 7.2, 30°C, recombinant wild-type enzyme | Corynebacterium glutamicum |