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Literature summary for 2.7.4.22 extracted from

  • Bucurenci, N.; Serina, L.; Zaharia, C.; Landais, S.; Danchin, A.; Barzu, O.
    Mutational analysis of UMP kinase from Escherichia coli (1998), J. Bacteriol., 180, 473-477.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
GTP most effective at pH 8 and a high (1 mM) UMP concentration Escherichia coli

Application

Application Comment Organism
drug development potential target for antibacterial drugs Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
D146N 84% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
D159N exhibits higher solubility than the wild-type protein at neutral pH, 10% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
D168N 97% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
D174N loss of two-thirds of its activity after 3 months storage at 4°C in 50 mM Tris-HCl (pH 7.4), 98% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
D201N exhibits higher solubility than the wild-type protein at neutral pH, 42% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
D77N 84% of wild-type activity in the pellet of the sonicated bacterial extract, insensitive to activation by GTP Escherichia coli
R62H 83% of wild-type activity in the pellet of the sonicated bacterial extract, insensitive to activation by GTP Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
GTP mutant D201N is inhibited by GTP above 0.2 mM Escherichia coli
UTP the inhibitory effect is maximal at pH 8 and a low (0.1 mM) UMP concentration Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ATP mutant D146N, at pH 6 Escherichia coli
0.043
-
UMP wild-type, at pH 8 Escherichia coli
0.048
-
ATP wild-type, at pH 6 Escherichia coli
0.052
-
UMP mutant D159N, at pH 8 Escherichia coli
0.059
-
UMP mutant D146N, at pH 8 Escherichia coli
0.06
-
UMP mutant D168N, at pH 8 Escherichia coli
0.095
-
ATP mutant D159N, at pH 6 Escherichia coli
0.1
-
ATP mutant D146N, at pH 8 Escherichia coli
0.12
-
ATP mutant D77N, at pH 6 Escherichia coli
0.12
-
ATP wild-type, at pH 8 Escherichia coli
0.14
-
ATP mutant D168N, at pH 6 Escherichia coli
0.17
-
ATP mutant D174N, at pH 6 Escherichia coli
0.17
-
UMP wild-type, at pH 6 Escherichia coli
0.2
-
ATP mutant D201N, at pH 6 Escherichia coli
0.22
-
UMP mutant D201N, at pH 8 Escherichia coli
0.24
-
ATP mutant R62H, at pH 6 Escherichia coli
0.27
-
UMP mutant D159N, at pH 6 Escherichia coli
0.29
-
ATP mutant D159N, at pH 8 Escherichia coli
0.31
-
ATP mutant D174N, at pH 8 Escherichia coli
0.31
-
UMP mutant D174N, at pH 8 Escherichia coli
0.32
-
UMP mutant D168N, at pH 6 Escherichia coli
0.32
-
UMP mutant R62H, at pH 8 Escherichia coli
0.42
-
ATP mutant D168N, at pH 8 Escherichia coli
0.45
-
UMP mutant D146N, at pH 6 Escherichia coli
0.46
-
ATP mutant D201N, at pH 8 Escherichia coli
0.47
-
UMP mutant D77N, at pH 8 Escherichia coli
0.48
-
ATP mutant D77N, at pH 8 Escherichia coli
0.86
-
UMP mutant D201N, at pH 6 Escherichia coli
1.26
-
UMP mutant R62H, at pH 6 Escherichia coli
1.78
-
UMP mutant D77N, at pH 6 Escherichia coli
3
-
ATP mutant R62H, at pH 8 Escherichia coli
3.7
-
UMP mutant D174N, at pH 6 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UMP Escherichia coli
-
ADP + UDP
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7E9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
mutant D146N, at pH 6 Escherichia coli
0.32
-
mutant D146N, at pH 8 Escherichia coli
1.04
-
mutant D201N, at pH 6 Escherichia coli
1.5
-
mutant R62H, at pH 6 Escherichia coli
1.9
-
mutant D77N, at pH 6 Escherichia coli
4.4
-
mutant R62H, at pH 8 Escherichia coli
8.2
-
mutant D201N, at pH 8 Escherichia coli
8.2
-
mutant D77N, at pH 8 Escherichia coli
32
-
mutant D174N, at pH 6 Escherichia coli
45
-
mutant D174N, at pH 8 Escherichia coli
63
-
mutant D168N, at pH 6 Escherichia coli
86.6
-
mutant D168N, at pH 8 Escherichia coli
105
-
wild-type, at pH 6 Escherichia coli
128
-
mutant D159N, at pH 6 Escherichia coli
128
-
wild-type, at pH 8 Escherichia coli
153
-
mutant D159N, at pH 8 Escherichia coli

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl (pH 7.4), several months, no significant loss of activity. An exception is mutant D174N, which loses two-thirds of its activity after 3 months at 4°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UMP
-
Escherichia coli ADP + UDP
-
?
ATP + UMP 30°C, pH 6, 7.4 or 8, 2 mM MgCl2 Escherichia coli ADP + UDP
-
?

Subunits

Subunits Comment Organism
hexamer alpha6, gel filtration Escherichia coli

Synonyms

Synonyms Comment Organism
UMP kinase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
Tm for mutant D201N Escherichia coli
52
-
Tm for mutant D77N Escherichia coli
59 62 mutants R62H, D146N, D168N and D174N are half inactivated between 59 and 62°C Escherichia coli
63
-
wild-type Tm Escherichia coli
73
-
Tm for mutant D159N Escherichia coli