Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
CMP | pH 7.4, 30°C | Escherichia coli | |
0.04 | - |
CMP | pH 7.4, 30°C | Bacillus subtilis | |
0.04 | - |
CMP | at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH | Bacillus subtilis | |
0.094 | - |
dCMP | pH 7.4, 30°C | Escherichia coli | |
0.12 | - |
ATP | at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH | Bacillus subtilis | |
0.33 | - |
dCMP | pH 7.4, 30°C | Bacillus subtilis | |
0.33 | - |
dCMP | at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH | Bacillus subtilis | |
0.36 | - |
araCMP | pH 7.4, 30°C | Escherichia coli | |
0.37 | - |
araCMP | pH 7.4, 30°C | Bacillus subtilis | |
0.37 | - |
ara-CMP | at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH | Bacillus subtilis | |
0.93 | - |
UMP | pH 7.4, 30°C | Escherichia coli | |
3.6 | - |
UMP | pH 7.4, 30°C | Bacillus subtilis | |
3.6 | - |
UMP | at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ara-CMP | - |
Escherichia coli | ADP + ara-CDP | - |
? | |
ATP + ara-CMP | ara-CMP is phosphorylated at rate which represents 14% of that with CMP | Bacillus subtilis | ADP + ara-CDP | - |
? | |
ATP + CMP | - |
Bacillus subtilis | ADP + CDP | - |
? | |
ATP + CMP | - |
Escherichia coli | ADP + CDP | - |
? | |
ATP + CMP | CMP and dCMP are the best phosphate acceptors | Bacillus subtilis | ADP + CDP | - |
? | |
ATP + dCMP | - |
Escherichia coli | ADP + dCDP | - |
? | |
ATP + dCMP | CMP and dCMP are the best phosphate acceptors | Bacillus subtilis | ADP + dCDP | - |
? | |
ATP + GTP | GTP is a poor substrate with Bacillus subtilis CMP kinase | Bacillus subtilis | ADP + GDP | - |
? | |
ATP + UMP | - |
Bacillus subtilis | ADP + UDP | - |
? | |
ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
? | |
ATP + UMP | UMP is phosphorylated at rate which represents 10% of that with CMP | Bacillus subtilis | ADP + UDP | - |
? | |
dATP + CMP | - |
Escherichia coli | dADP + CDP | - |
? | |
GTP + CMP | poor substrate | Escherichia coli | GDP + CDP | - |
? | |
GTP + CMP | ATP is equally effective as ATP | Bacillus subtilis | GDP + CDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMP kinase | - |
Bacillus subtilis |
deoxycytidine monophosphate kinase | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
midpoint denaturation temperature in absence of nucleotide substrates or in presence of ATP | Escherichia coli |
48.5 | 57.8 | the melting temperature of CMP kinae in the absence of nucleotide substrates is at 48.5°C, the melting temperature of CMP kinae in the presence of ATP is at 57.8°C, the melting temperature of CMP kinae in the presence of CMP is at 50.5°C | Bacillus subtilis |
49 | - |
midpoint denaturation temperature in presence of CMP | Escherichia coli |
49 | - |
midpoint denaturation temperature in absence of nucleotide substrates | Bacillus subtilis |
51 | - |
midpoint denaturation temperature in presence of CMP | Bacillus subtilis |
58 | - |
midpoint denaturation temperature in presence of ATP | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus subtilis |