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Literature summary for 2.7.4.3 extracted from
- Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani (2003), Eur. J. Biochem., 270, 4339-4347.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| P1,P5-(bis adenosine)-5'-pentaphosphate | inhibited activity of the recombinant enzyme, also inhibited the growth of L. donovani promastigotes in vitro | Leishmania donovani |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.074 | - |
AMP | pH 8 | Leishmania donovani | |
| 0.104 | - |
ATP | pH 8 | Leishmania donovani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
calculated from nucleotide sequence | Leishmania donovani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | Q9Y0E7 | 1S2D strain | - |
| Leishmania donovani 1S2D | Q9Y0E7 | 1S2D strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leishmania donovani |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| refolding of urea-denatured adenylate kinase by equilibrium dialysis against 50 mM Tris/HCl pH 8.0, containing either MgSO4, KCl or 10 mM dithiothreitol | Leishmania donovani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| promastigote | - |
Leishmania donovani | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Vmax 0.00018 pH 8 | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AMP | - |
Leishmania donovani | ADP + ADP | - |
? | |
| ATP + AMP | - |
Leishmania donovani 1S2D | ADP + ADP | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
t1/2: 29 h | Leishmania donovani |
| 13 | - |
t1/2: 24 h | Leishmania donovani |
| 26.5 | - |
t1/2: 12h | Leishmania donovani |
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Release 2023.1 (January 2023)
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