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Literature summary for 2.7.4.3 extracted from

  • Kinukawa, M.; Vacquier, V.D.
    Recombinant sea urchin flagellar adenylate kinase (2007), J. Biochem., 142, 501-506.
    View publication on PubMed
Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length protein and each of its three catalytic domains in Escherichia coli Strongylocentrotus purpuratus

Protein Variants

Protein Variants Comment Organism
additional information expression of full-length protein and each of its three catalytic domains in Escherichia coli. Full-length protein displays high catalytic activity, while none of the catalytic domains alone is active. Strongylocentrotus purpuratus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 51% inhibition at 1 mM Strongylocentrotus purpuratus
P1,P5-di(adenosine-5')pentaphosphate
-
 Strongylocentrotus purpuratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 ADP pH 7.6 Strongylocentrotus purpuratus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 x * 130000, SDS-PAGE Strongylocentrotus purpuratus

Organism

Organism UniProt Comment Textmining
Strongylocentrotus purpuratus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein enzyme has three cAMP-dependent protein kinase phosphorylation sites, and can be phosphorylated in vitro. The enzymatic kinetics after phosphorylation are not significantly altered Strongylocentrotus purpuratus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP
-
 Strongylocentrotus purpuratus AMP + ATP
-
 ?

Subunits

Subunits Comment Organism
? x * 130000, SDS-PAGE Strongylocentrotus purpuratus
More expression of each of the catalytic domains. SDS-PAGE reveals 22 kDa for catalytic domain 1, 21 kDa for catalyticdomain 2 and 22 kDa for catalytic domain 3 Strongylocentrotus purpuratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 native enzyme Strongylocentrotus purpuratus
8.1
-
 recombinant full-length protein Strongylocentrotus purpuratus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00053
-
 pH 7.6 Strongylocentrotus purpuratus P1,P5-di(adenosine-5')pentaphosphate