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Literature summary for 2.7.4.3 extracted from

  • Solaroli, N.; Panayiotou, C.; Johansson, M.; Karlsson, A.
    Identification of two active functional domains of human adenylate kinase 5 (2009), FEBS Lett., 583, 2872-2876.
    View publication on PubMed
Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.172
-
 AMP adenylate kinase 5 domain AK5p1, with ATP as phosphate donor Homo sapiens
2
-
 dAMP Km above 2.0 mM, adenylate kinase 5 domain AK5p1, with ATP as phosphate donor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
nucleus
-
 Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y6K8
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP adenylate kinase 5 domain AK5p1, at the assay conditions used, and at lower concentrations of substrate, AK5p1 shows generally a higher affinity for AMP compared to dAMP Homo sapiens ADP + ADP
-
 ?
ATP + CMP adenylate kinase 5 domain AK5p1, the relative efficiency of CMP is about 15% compared to AMP Homo sapiens ADP + CDP
-
 ?
ATP + dAMP adenylate kinase 5 domain AK5p1 Homo sapiens ADP + dADP
-
 ?
ATP + dCMP adenylate kinase 5 domain AK5p1, the relative efficiency of dCMP is about 15% compared to AMP Homo sapiens ADP + dCDP
-
 ?
additional information AK5p1 phosphorylates AMP, CMP, dAMP and dCMP with ATP or GTP as phosphate donors, AK5p2 phosphorylates AMP, CMP and dAMP when ATP is used as phosphate donor and AMP, CMP and dCMP with GTP as phosphate donor, AK5p2 cannot phosphorylate dAMP in the presence of GTP Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
adenylate kinase 5 isozyme, human AK5 has two separate functional domains both having enzymatic activity Homo sapiens
AK5 isozyme Homo sapiens
AK5p1 domain of adenylate kinase 5, human AK5 has two separate functional domains both having enzymatic activity Homo sapiens
AK5p2 domain of adenylate kinase 5, human AK5 has two separate functional domains both having enzymatic activity Homo sapiens