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Literature summary for 2.7.4.3 extracted from

  • Liang, P.; Zhang, F.; Chen, W.; Hu, X.; Huang, Y.; Li, S.; Ren, M.; He, L.; Li, R.; Li, X.; Xu, J.; Wu, Z.; Lu, G.; Yu, X.
    Identification and biochemical characterization of adenylate kinase 1 from Clonorchis sinensis (2013), Parasitol. Res., 112, 1719-1727.
    View publication on PubMed
Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clonorchis sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 ADP in 100 mM Tris–HCl (pH 7.5), 20 mM glucose, 5 mM MgCl2, 10 mM KCl, 2 mM dithiothreitol, at 37°C Clonorchis sinensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme is slightly activated by Ca2+ (85% relative activity at 5 mM) Clonorchis sinensis
K+ the enzyme is highly activated by K+, the optimal concentration is 10 mM Clonorchis sinensis
Li+ the enzyme is slightly activated by Li+ +(65.87% relative activity at 10 mM) Clonorchis sinensis
Mg2+ the enzyme activity is highly dependent on Mg2+, and the optimal concentration of Mg2+ is 2 mM Clonorchis sinensis
additional information the enzyme is not activated by Mn2+ Clonorchis sinensis
NH4+ the enzyme is highly activated by NH4+ (93.6% relative activity at 5 mM) Clonorchis sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26700
-
 x * 26700, His12-tagged enzyme ADK1, estimated from SDS-PAGE Clonorchis sinensis

Organism

Organism UniProt Comment Textmining
Clonorchis sinensis Q8MVS0
-
-

Purification (Commentary)

Purification (Comment) Organism
His Bind column chromatography Clonorchis sinensis

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Clonorchis sinensis
-
egg
-
 Clonorchis sinensis
-
metacercaria
-
 Clonorchis sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + ADP
-
 Clonorchis sinensis ATP + AMP
-
 r
ATP + AMP
-
 Clonorchis sinensis ADP + ADP
-
 r

Subunits

Subunits Comment Organism
? x * 26700, His12-tagged enzyme ADK1, estimated from SDS-PAGE Clonorchis sinensis

Synonyms

Synonyms Comment Organism
adenylate kinase 1
-
 Clonorchis sinensis
ADK1
-
 Clonorchis sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Clonorchis sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Clonorchis sinensis

Expression

Organism Comment Expression
Clonorchis sinensis the expression level of ADK1 at the stage of excysted metacercaria is higher than those of adult worm (18.8fold), metacercariae (1.5fold) and eggs (5.6fold) up