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Literature summary for 2.7.4.3 extracted from
- The adenylate kinases from a mesophilic and three thermophilic methanogenic members of the archaea (1995), J. Bacteriol., 177, 2977-2981.
General Stability
| General Stability | Organism |
|---|---|
| enzyme is fully active in air | Methanococcus voltae |
| enzyme is fully active in air | Methanotorris igneus |
| enzyme is fully active in air | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | 1 mM, complete inhibition of ADP-dependent ATP production | Methanococcus voltae |  |
| P1,P5-di(adenosine-5')pentaphosphate | - |
Methanocaldococcus jannaschii | |
| P1,P5-di(adenosine-5')pentaphosphate | 50% inhibition at 50 mM, whether assayed in the direction of ATP formation from ADP or of ATP conversion to ADP | Methanococcus voltae | |
| P1,P5-di(adenosine-5')pentaphosphate | - |
Methanothermococcus thermolithotrophicus | |
| P1,P5-di(adenosine-5')pentaphosphate | - |
Methanotorris igneus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Methanothermococcus thermolithotrophicus | 5829 | - |
| cytosol | - |
Methanococcus voltae | 5829 | - |
| cytosol | - |
Methanotorris igneus | 5829 | - |
| cytosol | - |
Methanocaldococcus jannaschii | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | maximum stimulation at 100 mM | Methanococcus voltae | |
| K+ | maximum stimulation at 100 mM | Methanocaldococcus jannaschii | |
| K+ | maximum stimulation at 200 mM | Methanotorris igneus | |
| K+ | maximum stimulation at 400 mM | Methanothermococcus thermolithotrophicus | |
| Mg2+ | activity is Mg2+ dependent | Methanococcus voltae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 25000, SDS-PAGE | Methanococcus voltae |
| 25000 | - |
x * 25000 (approximately), SDS-PAGE | Methanothermococcus thermolithotrophicus |
| 25000 | - |
x * 25000 (approximately), SDS-PAGE | Methanotorris igneus |
| 25000 | - |
x * 25000 (approximately), SDS-PAGE | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | P43409 | - |
- |
| Methanocaldococcus jannaschii DSM 2661 | P43409 | - |
- |
| Methanococcus voltae | P43411 | - |
- |
| Methanothermococcus thermolithotrophicus | P43410 | - |
- |
| Methanotorris igneus | P43408 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermococcus thermolithotrophicus |
- |
Methanococcus voltae |
- |
Methanotorris igneus |
- |
Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14 | - |
substrate: ATP, pH and temperature not specified in the publication | Methanococcus voltae |
| 56 | - |
substrate: ATP, pH and temperature not specified in the publication | Methanotorris igneus |
| 81 | - |
substrate: ATP, pH and temperature not specified in the publication | Methanothermococcus thermolithotrophicus |
| 89 | - |
substrate: ATP, pH and temperature not specified in the publication | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AMP | - |
Methanothermococcus thermolithotrophicus | 2 ADP | - |
r | |
| ATP + AMP | - |
Methanococcus voltae | 2 ADP | - |
r | |
| ATP + AMP | - |
Methanotorris igneus | 2 ADP | - |
r | |
| ATP + AMP | - |
Methanocaldococcus jannaschii | 2 ADP | - |
r | |
| ATP + AMP | - |
Methanocaldococcus jannaschii DSM 2661 | 2 ADP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE | Methanococcus voltae |
| ? | x * 25000 (approximately), SDS-PAGE | Methanothermococcus thermolithotrophicus |
| ? | x * 25000 (approximately), SDS-PAGE | Methanotorris igneus |
| ? | x * 25000 (approximately), SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Methanococcus voltae |
| 70 | - |
- |
Methanothermococcus thermolithotrophicus |
| 70 | - |
- |
Methanotorris igneus |
| 80 | 90 | - |
Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
1 h, the enzyme maintains 77.5% residual activity compared with the enzyme stored on ice | Methanococcus voltae |
| 59 | - |
1 h, the enzyme maintains 47% residual activity compared with the enzyme stored on ice | Methanococcus voltae |
| 68 | - |
t1/2: 30 min | Methanocaldococcus jannaschii |
| 68 | - |
t1/2: 4 min | Methanococcus voltae |
| 68 | - |
t1/2: 25 min | Methanothermococcus thermolithotrophicus |
| 68 | - |
t1/2: 43 min | Methanotorris igneus |
| 89 | - |
t1/2: 1.5 min | Methanococcus voltae |
| 89 | - |
t1/2: 17 min | Methanotorris igneus |
| 89 | - |
t1/2: 6.0 min | Methanothermococcus thermolithotrophicus |
| 89 | - |
t1/2: 6.0 min | Methanocaldococcus jannaschii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | enzyme shows similar activity over a pH range from 6 to 10 | Methanococcus voltae |
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