Literature summary for 2.7.4.33 extracted from

Kameda, A.; Shiba, T.; Kawazoe, Y.; Satoh, Y.; Ihara, Y.; Munekata, M.; Ishige, K.; Noguchi, T.
A novel ATP regeneration system using polyphosphate-AMP phosphotransferase and polyphosphate kinase (2001), J. Biosci. Bioeng., 91, 557-563.

Search for more literature for 2.7.4.33

Application

Application	Comment	Organism
synthesis	design of an ATP regeneration system with poylphosphate-AMP phosphotransferase and polyphosphate kinase. The ATP regeneration system can also be used as a GTP regeneration system	Myxococcus xanthus
synthesis	the phospho-conversion of AMP to ADP using polyphosphate as phosphate donor by polyphosphate-AMP phosphotransferase, PAP, followed by the synthesis of ATP from ADP through poylphosphate kinase, PPK, is used as an ATP regenrating system. The PAP-PPK ATP regeneration system can continously produce ATP in the coupled reaction from polyphosphate, whhich is a cheaper substrate than acetylphosphate, phosphoenolphosphate, or creatine phosphate. The system can also regenerate GTP from GMP. Method optimization, overview. ATP can be used for e.g. synthesis of acetly-CoA	Myxococcus xanthus
synthesis	the phospho-conversion of AMP to ADP using polyphosphate as phosphate donor by polyphosphate-AMP phosphotransferase, PAP, followed by the synthesis of ATP from ADP through poylphosphate kinase, PPK, is used as an ATP regenrating system. The PAP-PPK ATP regeneration system can continously produce ATP in the coupled reaction from polyphosphate, whhich is a cheaper substrate than acetylphosphate, phosphoenolphosphate, or creatine phosphate. The system can also regenerate GTP from GMP. Method optimization, overview. ATP can be used for e.g. synthesis of acetly-CoA	Acinetobacter johnsonii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Myxococcus xanthus	16020	-
soluble	-	Acinetobacter johnsonii	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Myxococcus xanthus
Mg2+	required	Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
AMP + [phosphate]n	Myxococcus xanthus	-	ADP + [phosphate]n-1	-	?
AMP + [phosphate]n	Acinetobacter johnsonii	-	ADP + [phosphate]n-1	-	?
AMP + [phosphate]n	Myxococcus xanthus DK101	-	ADP + [phosphate]n-1	-	?

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter johnsonii	-	-	-
Myxococcus xanthus	-	-	-
Myxococcus xanthus DK101	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 200fold by membrane fraction preparation and washing with detergent Triton X-100 and SB-12	Myxococcus xanthus
partial	Myxococcus xanthus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
12	-	partially purified native enzyme, pH 8.0, 37°C, ADP synthesis	Myxococcus xanthus

Storage Stability

Storage Stability	Organism
4°C, membrane fraction, over 2 months, stable	Myxococcus xanthus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AMP + (phosphate)n	-	Myxococcus xanthus	ADP + (phosphate)n-1	-	?
AMP + (phosphate)n	-	Myxococcus xanthus DK101	ADP + (phosphate)n-1	-	?
AMP + [phosphate]n	-	Myxococcus xanthus	ADP + [phosphate]n-1	-	?
AMP + [phosphate]n	-	Acinetobacter johnsonii	ADP + [phosphate]n-1	-	?
AMP + [phosphate]n	-	Myxococcus xanthus DK101	ADP + [phosphate]n-1	-	?
GMP + [phosphate]n	-	Myxococcus xanthus	GDP + [phosphate]n-1	-	?
GMP + [phosphate]n	-	Acinetobacter johnsonii	GDP + [phosphate]n-1	-	?
GMP + [phosphate]n	-	Myxococcus xanthus DK101	GDP + [phosphate]n-1	-	?

Synonyms

Synonyms	Comment	Organism
PAP	-	Myxococcus xanthus
PAP	-	Acinetobacter johnsonii
poylphosphate-AMP phosphotransferase	-	Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Myxococcus xanthus
37	-	assay at	Acinetobacter johnsonii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Myxococcus xanthus
8	-	assay at	Acinetobacter johnsonii

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Release 2023.1 (January 2023)