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Literature summary for 2.7.4.33 extracted from

  • Ishige, K.; Noguchi, T.
    Polyphosphate:AMP phosphotransferase and polyphosphate:ADP phosphotransferase activities of Pseudomonas aeruginosa (2001), Biochem. Biophys. Res. Commun., 281, 821-826.
    View publication on PubMed
Search for more literature for 2.7.4.33

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
AMP + [phosphate]n Pseudomonas aeruginosa best substrate ADP + [phosphate]n-1
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, separation from polyphosphate kinase activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + (phosphate)n the activity originates from combined action of the polyphosphate:ADP phosphotransferase and adenylate kinase Pseudomonas aeruginosa ADP + (phosphate)n-1
-
 ?
AMP + [phosphate]n best substrate Pseudomonas aeruginosa ADP + [phosphate]n-1
-
 ?
AMP + [phosphate]n best substrate is [phosphate]15 Pseudomonas aeruginosa ADP + [phosphate]n-1
-
 ?
additional information the partially purified polyphosphate:ADP phosphotransferase is independent of polyphosphate kinase, PPK, and can function as polyphosphate-dependent nucleoside diphosphate kinase, PADP, which most prefers GDP to the other three nucleoside diphosphates as a phospho-acceptor. Both of the polyphosphate-utilizing activities require short polyphosphate as a phospho-donor whose chain length is below 75. The PAP activity mainly originates from the combined action of adenylate kinase, ADK, and the PADP independent of PPK. Each partially purified enzyme alone cannot mediate phosphorylation of AMP with polyphosphate, but coincubation of the both enzymes results in a marked expression of PAP activity, confirming that PADP and ADK constitute the PAP Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
PADP
-
 Pseudomonas aeruginosa
PAP
-
 Pseudomonas aeruginosa
polyphosphate:ADP phosphotransferase
-
 Pseudomonas aeruginosa
polyphosphate:AMP phosphotransferase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Pseudomonas aeruginosa

General Information

General Information Comment Organism
additional information overexpression in strain PAO1 leads to a massive production of polyphosphate kinase, PPK, and a marked enhancement of polyphosphate-synthesizing activity, while the PAP activity is not affected by overproduction of PPK Pseudomonas aeruginosa