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Literature summary for 2.7.4.33 extracted from

  • Tanaka, S.; Kuroda, A.; Kato, J.; Ikeda, T.; Takiguchi, N.; Ohtake, H.
    A sensitive method for detecting AMP by utilizing polyphosphate-dependent ATP regeneration and bioluminescence reactions (2001), Biochem. Eng. J., 9, 193-197.
No PubMed abstract available
Search for more literature for 2.7.4.33

Application

Application Comment Organism
analysis sensitive method for detecting AMP by using polyphosphate(polyP)-AMP phosphotransferase and adenylate kinase from Acinetobacter johnsonii in conjugation with firefly luciferase. The method allows detection of AMP over the concentration range of 0.3-100 pmol per assay Acinetobacter johnsonii
diagnostics AMP is known to have potential for use as a reliable indicator in hygiene monitoring, the development of a sensitive method for detecting AMP, by using polyphosphate-AMP phosphotransferase and adenylate kinase in conjugation with firefly luciferase, is useful to detect food samples with high sensitivity Acinetobacter johnsonii
food industry AMP is known to have potential for use as a reliable indicator in hygiene monitoring, the development of a sensitive method for detecting AMP, by using polyphosphate-AMP phosphotransferase and adenylate kinase in conjugation with firefly luciferase, is useful to detect food samples with high sensitivity Acinetobacter johnsonii
additional information AMP is known to have potential for use as a reliable indicator in hygiene monitoring, the development of a sensitive method for detecting AMP, by using polyphosphate-AMP phosphotransferase and adenylate kinase in conjugation with firefly luciferase, is useful to detect food samples with high sensitivity Acinetobacter johnsonii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
AMP + [phosphate]n Acinetobacter johnsonii
-
 ADP + [phosphate]n-1
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter johnsonii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter johnsonii
native enzyme 20.1fold by anion exchange, hydrophobic interaction, and affinity chromatography Acinetobacter johnsonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00957
-
 pH and temperature not specified in the publication Acinetobacter johnsonii
9570
-
 purified native enzyme, pH 7.4, 30°C Acinetobacter johnsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + [phosphate]n
-
 Acinetobacter johnsonii ADP + [phosphate]n-1
-
 ?
additional information development of a sensitive method for detecting AMP by using polyphosphate-AMP phosphotransferase, PPT, and adenylate kinase, ADK, from Acinetobacter johnsonii in conjugation with firefly luciferase, polyP65 is used as substrate with AMP and Mg2+, overview Acinetobacter johnsonii ?
-
 ?

Synonyms

Synonyms Comment Organism
polyP-AMP phosphotransferase
-
 Acinetobacter johnsonii
polyphosphate(polyP)-AMP phosphotransferase
-
 Acinetobacter johnsonii
polyphosphate-AMP phosphotransferase
-
 Acinetobacter johnsonii
PPT
-
 Acinetobacter johnsonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Acinetobacter johnsonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Acinetobacter johnsonii