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Literature summary for 2.7.4.33 extracted from
- Properties of polyphosphate:AMP phosphotransferase of Acinetobacter strain 210A (1991), J. Bacteriol., 173, 6484-6488.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 6 mM, 30% inhibition | Acinetobacter sp. |  |
| diphosphate | - |
Acinetobacter johnsonii | |
| diphosphate | - |
Acinetobacter sp. | |
| EDTA | - |
Acinetobacter johnsonii | |
| Tetraphosphate | - |
Acinetobacter johnsonii | |
| Tetraphosphate | mixed type inhibition with polyphosphate as substrate | Acinetobacter sp. | |
| Triphosphate | - |
Acinetobacter johnsonii | |
| Triphosphate | mixed type inhibition with polyphosphate as substrate | Acinetobacter sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
(Phosphate)n | pH and temperature not specified in the publication, polyphosphate with an average chain length of 35 phosphate groups | Acinetobacter sp. | |
| 0.0008 | - |
[phosphate]n | polyphosphate with an average chain length of 35 phosphate groups, pH 8.5, 30°C | Acinetobacter johnsonii | |
| 0.26 | - |
AMP | pH 8.5, 30°C | Acinetobacter johnsonii | |
| 0.26 | - |
AMP | pH and temperature not specified in the publication | Acinetobacter sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Acinetobacter johnsonii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Acinetobacter sp. |
| 55000 | - |
x * 55000, SDS-PAGE | Acinetobacter johnsonii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + [phosphate]n | Acinetobacter johnsonii | - |
ADP + [phosphate]n-1 | - |
? | |
| AMP + [phosphate]n | Acinetobacter johnsonii 210A | - |
ADP + [phosphate]n-1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter johnsonii | - |
- |
- |
| Acinetobacter johnsonii 210A | - |
- |
- |
| Acinetobacter sp. | - |
- |
- |
| Acinetobacter sp. 210A | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acinetobacter sp. |
| native enzyme 1511fold from strain 210A by streptomycin sulfate precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Acinetobacter johnsonii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ADP + (phosphate)n = AMP + (phosphate)n+1 | the mechanism of polyphosphate degradation by polyphosphate:AMP phosphotransferase is processive. Once bound, the polyphosphate chain does not dissociate from the enzyme until it has been utilized completely | Acinetobacter johnsonii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 101.2 | - |
purified native enzyme, pH 8.5, 30°C | Acinetobacter johnsonii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1 month, about 50% loss of activity which is not due to freezing and thawing | Acinetobacter sp. |
| -20°C, purified enzyme, 1 month, 50% activity remaining, activity loss is not due to freezing and thawing | Acinetobacter johnsonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + (phosphate)n | highest activity are found with polyphosphate molecules of 18 to 44 phosphate residues. The polyphosphate chain is degraded completely to ADP. The mechanism of the degradation is processive. No activity is obtained with ortho-, di-, tri-, and tetraphosphate | Acinetobacter sp. | ADP + (phosphate)n-1 | - |
? | |
| AMP + (phosphate)n | highest activity are found with polyphosphate molecules of 18 to 44 phosphate residues. The polyphosphate chain is degraded completely to ADP. The mechanism of the degradation is processive. No activity is obtained with ortho-, di-, tri-, and tetraphosphate | Acinetobacter sp. 210A | ADP + (phosphate)n-1 | - |
? | |
| AMP + [phosphate]n | - |
Acinetobacter johnsonii | ADP + [phosphate]n-1 | - |
? | |
| AMP + [phosphate]n | polyphosphate with an average chain length of 35 phosphate groups is the best substrate | Acinetobacter johnsonii | ADP + [phosphate]n-1 | - |
? | |
| AMP + [phosphate]n | - |
Acinetobacter johnsonii 210A | ADP + [phosphate]n-1 | - |
? | |
| AMP + [phosphate]n | polyphosphate with an average chain length of 35 phosphate groups is the best substrate | Acinetobacter johnsonii 210A | ADP + [phosphate]n-1 | - |
? | |
| additional information | no activity with ortho-, di-, tri-, and tetraphosphates, highest activities with polyphosphate of a degree of polymerization between 18 and 44 phosphate groups, overview | Acinetobacter johnsonii | ? | - |
? | |
| additional information | no activity with ortho-, di-, tri-, and tetraphosphates, highest activities with polyphosphate of a degree of polymerization between 18 and 44 phosphate groups, overview | Acinetobacter johnsonii 210A | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Acinetobacter sp. |
| ? | x * 55000, SDS-PAGE | Acinetobacter johnsonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Acinetobacter johnsonii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
2 h, stable | Acinetobacter sp. |
| 30 | - |
purified enzyme, 2 h, completely stable | Acinetobacter johnsonii |
| 45 | - |
15 min, 50% loss of activity | Acinetobacter sp. |
| 45 | - |
purified enzyme, 15 min, loss of 50% activity | Acinetobacter johnsonii |
| 50 | - |
15 min, complete loss of activity | Acinetobacter sp. |
| 50 | - |
purified enzyme, inactivation | Acinetobacter johnsonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Acinetobacter sp. |
| 6.5 | 8.5 | - |
Acinetobacter johnsonii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | no activity is observed below pH 5.5 and above pH 10 | Acinetobacter sp. |
| 5.5 | 10 | no activity below pH 5.5 and above pH 10.0 | Acinetobacter johnsonii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics, overview | Acinetobacter johnsonii |
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