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Literature summary for 2.7.4.8 extracted from

  • Hible, G.; Renault, L.; Schaeffer, F.; Christova, P.; Zoe Radulescu, A.; Evrin, C.; Gilles, A.M.; Cherfils, J.
    Calorimetric and crystallographic analysis of the oligomeric structure of Escherichia coli GMP kinase (2005), J. Mol. Biol., 352, 1044-1059.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BLI5 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23460
-
monomer, electrospray ionization-mass spectrometry Escherichia coli
23462
-
x * 23462, thermodynamic analysis, the enzyme is in equilibrium between a dimer and higher order oligomers, whose relative amounts depend on protein concentration, ionic strength, and the presence of ATP Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + dGMP Escherichia coli
-
ADP + dGDP
-
r
ATP + GMP Escherichia coli
-
ADP + GDP
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P60546
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant ecGMPK is purified by a two-step chromatography procedure involving affinity chromatography on Blue Sepharose and gel filtration Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
complete denaturation of the protein is not reversible due to aggregation at the unfolded state Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, stable for several months after purification by Blue Sepharose and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dGMP
-
Escherichia coli ADP + dGDP
-
r
ATP + GMP
-
Escherichia coli ADP + GDP
-
r

Subunits

Subunits Comment Organism
hexamer crystallography Escherichia coli
oligomer x * 23462, thermodynamic analysis, the enzyme is in equilibrium between a dimer and higher order oligomers, whose relative amounts depend on protein concentration, ionic strength, and the presence of ATP Escherichia coli

Synonyms

Synonyms Comment Organism
ecGMPK
-
Escherichia coli
GMP kinase
-
Escherichia coli
GMPK
-
Escherichia coli