Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BLI5 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23460 | - |
monomer, electrospray ionization-mass spectrometry | Escherichia coli |
23462 | - |
x * 23462, thermodynamic analysis, the enzyme is in equilibrium between a dimer and higher order oligomers, whose relative amounts depend on protein concentration, ionic strength, and the presence of ATP | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dGMP | Escherichia coli | - |
ADP + dGDP | - |
r | |
ATP + GMP | Escherichia coli | - |
ADP + GDP | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60546 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant ecGMPK is purified by a two-step chromatography procedure involving affinity chromatography on Blue Sepharose and gel filtration | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
complete denaturation of the protein is not reversible due to aggregation at the unfolded state | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, stable for several months after purification by Blue Sepharose and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dGMP | - |
Escherichia coli | ADP + dGDP | - |
r | |
ATP + GMP | - |
Escherichia coli | ADP + GDP | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | crystallography | Escherichia coli |
oligomer | x * 23462, thermodynamic analysis, the enzyme is in equilibrium between a dimer and higher order oligomers, whose relative amounts depend on protein concentration, ionic strength, and the presence of ATP | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ecGMPK | - |
Escherichia coli |
GMP kinase | - |
Escherichia coli |
GMPK | - |
Escherichia coli |