Application | Comment | Organism |
---|---|---|
drug development | potential target for the development of new antituberculosis drugs due to the low sequence similarity with the human enzyme | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-bromo-2'-deoxyuridine | - |
Homo sapiens | |
5-bromo-2'-deoxyuridine | potential inhibitor with high selectivity between bacterial and human enzyme | Mycobacterium tuberculosis | |
5-chloro-2'-deoxyuridine | - |
Homo sapiens | |
5-chloro-2'-deoxyuridine | potential inhibitor with high selectivity between bacterial and human enzyme | Mycobacterium tuberculosis | |
5-fluoro-2'-deoxyuridine | not inhibitory at 4 mM | Homo sapiens | |
5-fluoro-2'-deoxyuridine | potential inhibitor with high selectivity between bacterial and human enzyme | Mycobacterium tuberculosis | |
5-iodo-2'-deoxyuridine | - |
Homo sapiens | |
5-iodo-2'-deoxyuridine | potential inhibitor with high selectivity between bacterial and human enzyme | Mycobacterium tuberculosis | |
deoxythymidine | - |
Homo sapiens | |
deoxythymidine | potential inhibitor with high selectivity between bacterial and human enzyme | Mycobacterium tuberculosis | |
dGMP | competitive | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
dTMP | pH 7.4, 30°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dTMP | Homo sapiens | involved in dTTP metabolism, essential for cell proliferation | ADP + dTDP | - |
? | |
ATP + dTMP | Mycobacterium tuberculosis | involved in dTTP metabolism, essential for cell proliferation | ADP + dTDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dTMP | - |
Homo sapiens | ADP + dTDP | - |
? | |
ATP + dTMP | - |
Mycobacterium tuberculosis | ADP + dTDP | - |
? | |
ATP + dTMP | involved in dTTP metabolism, essential for cell proliferation | Homo sapiens | ADP + dTDP | - |
? | |
ATP + dTMP | involved in dTTP metabolism, essential for cell proliferation | Mycobacterium tuberculosis | ADP + dTDP | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
5-bromo-2'-deoxyuridine | pH 7.4, 30°C | Mycobacterium tuberculosis | |
0.01 | - |
5-chloro-2'-deoxyuridine | pH 7.4, 30°C | Mycobacterium tuberculosis | |
0.027 | - |
deoxythymidine | pH 7.4, 30°C | Mycobacterium tuberculosis | |
0.033 | - |
5-iodo-2'-deoxyuridine | pH 7.4, 30°C | Mycobacterium tuberculosis | |
0.117 | - |
dGMP | pH 7.4, 30°C | Mycobacterium tuberculosis | |
0.18 | - |
deoxythymidine | pH 7.4, 30°C | Homo sapiens | |
0.212 | - |
5-fluoro-2'-deoxyuridine | pH 7.4, 30°C | Mycobacterium tuberculosis | |
0.214 | - |
5-bromo-2'-deoxyuridine | pH 7.4, 30°C | Homo sapiens | |
0.35 | - |
5-iodo-2'-deoxyuridine | pH 7.4, 30°C | Homo sapiens | |
0.375 | - |
5-chloro-2'-deoxyuridine | pH 7.4, 30°C | Homo sapiens |