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Literature summary for 2.7.6.1 extracted from
- Rat liver phosphoribosyl pyrophosphate synthetase: existence of the purified enzyme as heterogeneous aggregates and identification of the catalytic subunit (1989), J. Biochem., 105, 736-741.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
purified enzyme appears to exist as complex aggregates composed of heterogeneous components | Rattus norvegicus |
| 34000 | - |
x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit | Rattus norvegicus |
| 38000 | - |
x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit | Rattus norvegicus |
| 40000 | - |
x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit | Rattus norvegicus |
| 1000000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| polyethylene glycol precipitation, hydroxyapatite fractionation, gel filtration on Toyopearl HW-65F, chromatography on DEAE-Toyopearl 650S and gel filtration | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.28 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80ºC, potassium phosphate buffer, pH 7.4, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit | Rattus norvegicus |
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