Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | isoenzymes PRS1 and PRS2 require phosphate for activity, isoenzymes PRS3 and PRS4 are phosphate independent | Spinacia oleracea |
Cloned (Comment) | Organism |
---|---|
expression of isoenzymes PRS1, PRS2, PRS3 and PRS4 in Escherichia coli | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibits isoenzymes PRS1 and PRS2 efficiently, but not isoenzymes PRS3 and PRS4 | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
mitochondrion | - |
Spinacia oleracea | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Spinacia oleracea | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
mitochondria preparation | Spinacia oleracea |
0.004 | - |
chloroplast preparation | Spinacia oleracea |
0.014 | - |
isoenzyme PRS1, without phosphate and ADP | Spinacia oleracea |
0.018 | - |
isoenzyme PRS2, in the presence of phosphate 50 mM, and ADP 1 mM | Spinacia oleracea |
0.025 | - |
isoenzyme PRS2, without phosphate and ADP | Spinacia oleracea |
0.072 | - |
isoenzyme PRS4, in the presence of phosphate 50 mM, and ADP 1 mM | Spinacia oleracea |
0.074 | - |
isoenzyme PRS1, in the presence of phosphate 50 mM, and ADP 1 mM | Spinacia oleracea |
0.08 | - |
isoenzyme PRS4, in the presence of phosphate 50 mM, without ADP | Spinacia oleracea |
0.089 | - |
isoenzyme PRS4, without phosphate and ADP | Spinacia oleracea |
0.099 | - |
isoenzyme PRS4, in the presence of ADP 1 mM, without phosphate | Spinacia oleracea |
0.14 | - |
isoenzyme PRS2, in the presence of phosphate 50 mM, without ADP | Spinacia oleracea |
0.191 | - |
isoenzyme PRS1, in the presence of phosphate 50 mM, without ADP | Spinacia oleracea |
0.88 | - |
isoenzyme PRS3, in the presence of phosphate 50 mM, and ADP 1 mM | Spinacia oleracea |
0.884 | - |
isoenzyme PRS3, in the presence of ADP 1 mM, without phosphate | Spinacia oleracea |
1.06 | - |
isoenzyme PRS3, in the presence of phosphate 50 mM, without ADP | Spinacia oleracea |
1.26 | - |
isoenzyme PRS3, without phosphate and ADP | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Spinacia oleracea | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Spinacia oleracea |